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Free Fatty Acids (FFA) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS285613

Intended Uses: For the quantitative detection of Human Fibroblast Growth Factor 8 (FGF8) concentration in serum, plasma and other biological fluids.Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate FGF8 in Human serum, plasma.… More details

$545 / 48 Tests +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Free fatty acids (FFA), ELISA Kit
Full Product Name
Human Free fatty acids (FFA) ELISA Kit
Product Gene Name
FFA elisa kit
Species Reactivity
Human
Assay Type
Quantitative Sandwich
Samples
Serum, Plasma And Other Biological Fluids
Detection Range
1.56 pg/mL-100 pg/mL
Sensitivity
1 pg/mL (mean of 6 independent assays).
Specificity
This assay has high sensitivity and excellent specificity for detection of Human FGF8. No significant cross-reactivity or interference between Human FGF8 and analogues was observed.
Preparation and Storage
Store at 2-8 degree C.
Inter-assay Precision
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
Intra-assay Precision
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Molecular Weight
31,457 Da
OMIM
603820
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of FFA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FFA purchase

MBS285613 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Free fatty acids (FFA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FFA. The ELISA analytical biochemical technique of the MBS285613 kit is based on FFA antibody-FFA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FFA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FFA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for FFA. It may not necessarily be applicable to this product.

NCBI GI #
4885327
NCBI GeneID
2864
NCBI Accession #
NP_005294.1
UniProt Secondary Accession #
Q0VAS2; Q4VBL4
UniProt Related Accession #
O14842
GenBank Nucleotide #
NM_005303.2
NCBI Official Full Name
free fatty acid receptor 1
NCBI Official Synonym Full Names
free fatty acid receptor 1
NCBI Official Symbol
FFAR1
NCBI Official Synonym Symbols
FFA1R; GPR40; GPCR40
UniProt Protein Name
Free fatty acid receptor 1
UniProt Synonym Protein Names
G-protein coupled receptor 40
UniProt Gene Name
FFAR1
UniProt Synonym Gene Names
GPR40
UniProt Entry Name
FFAR1_HUMAN
NCBI Protein Information
free fatty acid receptor 1

NCBI Summary for FFA

This gene encodes a member of the GP40 family of G protein-coupled receptors that are clustered together on chromosome 19. The encoded protein is a receptor for medium and long chain free fatty acids and may be involved in the metabolic regulation of insulin secretion. Polymorphisms in this gene may be associated with type 2 diabetes. [provided by RefSeq, Apr 2009]
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UniProt Comments for FFA

FFAR1: Receptor for medium and long chain saturated and unsaturated fatty acids. Binding of the ligand increase intracellular calcium concentration and amplify glucose-stimulated insulin secretion. The activity of this receptor is mediated by G- proteins that activate phospholipase C. Seems to act through a G(q) and G(i)-mediated pathway. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: bioactive lipid receptor activity; G-protein coupled receptor activity; guanyl-nucleotide exchange factor activity; lipid binding

Biological Process: energy reserve metabolic process; G-protein coupled receptor protein signaling pathway; glucose homeostasis; insulin secretion; positive regulation of calcium ion transport; positive regulation of GTPase activity; positive regulation of insulin secretion; regulation of insulin secretion
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Research Articles on FFA

1. upregulation of GPR40 expression enhances the mitogenic response to epoxyeicosatrienoic acids

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with FFA elisa kit

 Reference Product  PubMed Publications
 FFAR3 elisa kit  >20 publications with FFA and FFAR3
 FFAR2 elisa kit  >9 publications with FFA and FFAR2
 CCK elisa kit  >6 publications with FFA and CCK
 GIP elisa kit  >5 publications with FFA and GIP

Pathways associated with FFA elisa kit

 Products by Pathway  Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Fatty Acids Bound To GPR40 (FFAR1) Regulate Insulin Secretion Pathway antibodies  Fatty Acids Bound To GPR40 (FFAR1) Regulate Insulin Secretion Pathway Diagram
 Free Fatty Acid Receptors Pathway antibodies  Free Fatty Acid Receptors Pathway Diagram
 Free Fatty Acids Regulate Insulin Secretion Pathway antibodies  Free Fatty Acids Regulate Insulin Secretion Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 GPCRs, Class A Rhodopsin-like Pathway antibodies  GPCRs, Class A Rhodopsin-like Pathway Diagram
 Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies  Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
 Incretin Synthesis, Secretion, And Inactivation Pathway antibodies  Incretin Synthesis, Secretion, And Inactivation Pathway Diagram

Diseases associated with FFA elisa kit

 Disease Name  Pubmed Publications
 Diabetes Mellitus, Type 2 Antibodies  >46 publications with FFA and Diabetes Mellitus, Type 2
 Insulin Resistance Antibodies  >21 publications with FFA and Insulin Resistance
 Hypoglycemia Antibodies  >15 publications with FFA and Hypoglycemia
 Fatty Liver Antibodies  >8 publications with FFA and Fatty Liver
 Hyperglycemia Antibodies  >6 publications with FFA and Hyperglycemia
 Diabetes Mellitus, Experimental Antibodies  >6 publications with FFA and Diabetes Mellitus, Experimental
 Inflammation Antibodies  >6 publications with FFA and Inflammation
 Kidney Diseases Antibodies  >4 publications with FFA and Kidney Diseases
 Necrosis Antibodies  >3 publications with FFA and Necrosis
 Weight Loss Antibodies  >1 publications with FFA and Weight Loss

Organs/Tissues associated with FFA elisa kit

 Organ/Tissue Name  Pubmed Publications
 Pancreas Antibodies  >48 publications with FFA and Pancreas
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