Br Serine/Threonine-Protein Kinase 1 (BRSK1) ELISA Kit for Mouse

Name: Mouse BRSK1 ELISA Kit; Br Serine/Threonine-Protein Kinase 1; MyBioSource
Brand: MyBioSource
SKU: MBS285635
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS285635

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate BRSK1 in samples. An antibody specific for BRSK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyBRSK1 present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

BR serine/threonine-protein kinase 1 (BRSK1), ELISA Kit

Full Product Name

Mouse BR serine/threonine-protein kinase 1 (BRSK1) ELISA Kit

Product Gene Name

BRSK1 elisa kit

Product Synonym Names

SAD1; SADB; Brain-specific serine/threonine-protein kinase 1; Synapses of Amphids Defective homolog 1; Serine/threonine-protein kinase BRSK1; BR serine/threonine kinase 1

Species Reactivity

Mouse

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Mouse BRSK1. No significant cross-reactivity or interference between Mouse BRSK1 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

35,121 Da

3D Structure

ModBase 3D Structure for Q5RJI5

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of BRSK1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BRSK1 purchase

MBS285635 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the BR serine/threonine-protein kinase 1 (BRSK1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing BRSK1. The ELISA analytical biochemical technique of the MBS285635 kit is based on BRSK1 antibody-BRSK1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BRSK1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BRSK1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for BRSK1. It may not necessarily be applicable to this product.

NCBI GI #

83649715

NCBI GeneID

381979

NCBI Accession #

NP_001003920.2

UniProt Primary Accession #

Q5RJI5

UniProt Secondary Accession #

Q699J6; A7LH90; A7LH91; B7SRN7

UniProt Related Accession #

Q5RJI5

GenBank Nucleotide #

NM_001003920.3

NCBI Official Full Name

serine/threonine-protein kinase BRSK1 isoform 1

NCBI Official Synonym Full Names

BR serine/threonine kinase 1

NCBI Official Symbol

Brsk1

NCBI Official Synonym Symbols

SADB; SAD-B; Gm1100

UniProt Protein Name

Serine/threonine-protein kinase BRSK1

UniProt Synonym Protein Names

Brain-specific serine/threonine-protein kinase 1; BR serine/threonine-protein kinase 1; Serine/threonine-protein kinase SAD-B

Protein Family

Serine/threonine-protein kinase

UniProt Gene Name

Brsk1

UniProt Synonym Gene Names

Gm1100; Sadb; BR serine/threonine-protein kinase 1

NCBI Protein Information

serine/threonine-protein kinase BRSK1

UniProt Comments for BRSK1

BRSK1: a serine/threonine protein kinase of the CAMK group. Closely related to AMPK. Activated by the kinase LKB1. Acts as checkpoint kinase upon DNA damage induced by UV or methyl methane sulfonate. Phosphorylates WEE1 and CDC25B in vitro and CDC25C in vitro and in vivo. Translocates from the cytoplasm to the nucleus in response to UV- or MMS-induced DNA damage. BRSK1 and BRSK2 are required for neuronal polarization. Widely expressed with highest levels in the brain and testis. Two alternatively spliced isoforms have been described.

Protein type: BRSK subfamily; CAMK group; CAMKL family; EC 2.7.11.1; EC 2.7.11.26; Kinase, protein; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 7|7 A1

Cellular Component: cell junction; centrosome; cytoplasm; cytoskeleton; nucleoplasm; nucleus; synapse; synaptic vesicle

Molecular Function: ATP binding; gamma-tubulin binding; kinase activity; magnesium ion binding; metal ion binding; nucleotide binding; protein binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity; tau-protein kinase activity; transferase activity

Biological Process: axonogenesis; cell cycle; cellular response to DNA damage stimulus; centrosome duplication; establishment of cell polarity; G2 DNA damage checkpoint; G2/M transition of mitotic cell cycle; nervous system development; neuron differentiation; neuron projection morphogenesis; neurotransmitter secretion; peptidyl-serine phosphorylation; phosphorylation; protein amino acid phosphorylation; response to UV

Research Articles on BRSK1

1. CAST/SAD-B reaction serves as a brake on synaptic transmission

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with BRSK1 elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>10 publications with BRSK1 and Brain