Progesterone (PROG) ELISA Kit for Sheep

Progesterone is a steroid hormone which plays an important role in the preparation for and maintenance of pregnancy. It is synthesized from cholesterol via pregnenolone, then rapidly metabolized to pregnanediol, for the most part, in the liver. The ovary and placenta are the major production sites; but a small amount is also synthesized by the adrenal cortex in both men and women. Circulating progesterone levels, which are characteristically low during the follicular phase, increase sharply during the luteal phase of menstrual cycles, reaching a maximum some 5 to 10 days after the midcycle LH peak. Unless pregnancy occurs, a steep decline to follicular levels sets in about 4 days before the next menstrual period.Progesterone is produced in the ovaries (specifically after ovulation in the corpus luteum), the adrenal glands (near the kidney), and, during pregnancy, in the placenta. Progesterone is also stored in adipose (fat) tissue.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PROG in samples. An antibody specific for PROG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPROG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PROG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PROG bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of PROG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS285645 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Progesterone, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PROG. The ELISA analytical biochemical technique of the MBS285645 kit is based on PROG antibody-PROG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PROG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PROG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).