Tryptase Gamma (TPSG1) ELISA Kit for Mouse

Name: Mouse TPSG1 ELISA Kit; Tryptase Gamma; MyBioSource
Brand: MyBioSource
SKU: MBS285654
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS285654

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate TPSG1 in samples. An antibody specific for TPSG1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTPSG1 present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Tryptase gamma (TPSG1), ELISA Kit

Full Product Name

Mouse Tryptase gamma (TPSG1) ELISA Kit

Product Gene Name

TPSG1 elisa kit

Product Synonym Names

Species Reactivity

Mouse

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Mouse TPSG1. No significant cross-reactivity or interference between Mouse TPSG1 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

32,656 Da

3D Structure

ModBase 3D Structure for Q9QUL7

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of TPSG1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TPSG1 purchase

MBS285654 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Tryptase gamma (TPSG1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TPSG1. The ELISA analytical biochemical technique of the MBS285654 kit is based on TPSG1 antibody-TPSG1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TPSG1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TPSG1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for TPSG1. It may not necessarily be applicable to this product.

NCBI GI #

18203386

NCBI Accession #

Q9QUL7.1

UniProt Primary Accession #

Q9QUL7

NCBI Official Full Name

Tryptase gamma

UniProt Protein Name

Tryptase gamma

UniProt Synonym Protein Names

Transmembrane tryptase

Protein Family

Tryptase

UniProt Gene Name

Tpsg1

UniProt Synonym Gene Names

Tmt

UniProt Comments for TPSG1

TPSG1: Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. There is uncertainty regarding the number of genes in this cluster. Currently four functional genes - alpha I, beta I, beta II and gamma I - have been identified. And beta I has an allelic variant named alpha II, beta II has an allelic variant beta III, also gamma I has an allelic variant gamma II. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha-tryptases predominant. This gene differs from other members of the tryptase gene family in that it has C-terminal hydrophobic domain, which may serve as a membrane anchor. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. [provided by RefSeq, Jul 2008]

Protein type: EC 3.4.21.-; Membrane protein, integral; Protease

Chromosomal Location of Human Ortholog: 17 A3.3|17 12.53 cM

Cellular Component: integral component of membrane; membrane

Molecular Function: hydrolase activity; peptidase activity; serine-type endopeptidase activity; serine-type peptidase activity

Biological Process: proteolysis

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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