Deoxyhypusine Hydroxylase (DOHH) ELISA Kit for Mouse

DOHH encodes a metalloenzyme that catalyzes the last step in the conversion of lysine to the unique amino acid hypusine in eukaryotic initiation factor 5A. The encoded protein hydroxylates deoxyhypusine to form hypusine in the mature eukaryotic initiation factor 5A protein. Alternative splicing results in multiple transcript variants.
DOHH contains 8 heat repeats organized in a symmetrical dyad connected by a variable region. It contains 2 potential metal coordination sites, each composed of 2 strictly conserved his-glu motifs.CD spectroscopy of purified recombinant wildtype DOHH indicated an alpha-helix-rich protein,and site-directed mutagenesis showed that the alpha-helical structure of DOHH does not depend on the conserved his-glu motifs or metal binding.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate DOHH in samples. An antibody specific for DOHH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDOHH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DOHH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DOHH bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of DOHH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS285669 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Deoxyhypusine hydroxylase (DOHH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DOHH. The ELISA analytical biochemical technique of the MBS285669 kit is based on DOHH antibody-DOHH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DOHH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DOHH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).