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Myogenin (MYOG) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS285673

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MYOG in samples. An antibody specific for MYOG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYOG present is bound by the… More details

$545 / 48 Tests +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Myogenin, ELISA Kit
Full Product Name
Human Myogenin (MYOG) ELISA Kit
Product Gene Name
MYOG elisa kit
Product Synonym Names
MYF4, MYOGENIN, bHLHc3, Myogenic factor-4; myogenin|myogenic factor 4|myogenin
Species Reactivity
Human
Assay Type
Sandwich
Samples
Serum, Plasma, Other biological fluids
Specificity
This assay has high sensitivity and excellent specificity for detection of Human MYOG. No significant cross-reactivity or interference between Human MYOG and analogues was observed.
Preparation and Storage
Store at 2-8 degree C.
Molecular Weight
25,037 Da
OMIM
X17651 mRNA
3D Structure
ModBase 3D Structure for P15173
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of MYOG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MYOG purchase

MBS285673 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Myogenin, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MYOG. The ELISA analytical biochemical technique of the MBS285673 kit is based on MYOG antibody-MYOG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MYOG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MYOG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for MYOG. It may not necessarily be applicable to this product.

NCBI GI #
18765727
NCBI GeneID
4656
NCBI Accession #
NP_002470.2
UniProt Primary Accession #
P15173
UniProt Secondary Accession #
Q53XW6
UniProt Related Accession #
P15173
GenBank Nucleotide #
NM_002479.5
NCBI Official Full Name
myogenin
NCBI Official Synonym Full Names
myogenin
NCBI Official Symbol
MYOG
NCBI Official Synonym Symbols
MYF4; myf-4; bHLHc3
UniProt Protein Name
Myogenin
UniProt Synonym Protein Names
Class C basic helix-loop-helix protein 3; bHLHc3; Myogenic factor 4; Myf-4
Protein Family
Myogenin
UniProt Gene Name
MYOG
UniProt Synonym Gene Names
BHLHC3; MYF4; bHLHc3; Myf-4
NCBI Protein Information
myogenin

NCBI Summary for MYOG

Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture. It is a member of a large family of proteins related by sequence homology, the helix-loop-helix (HLH) proteins. It is essential for the development of functional skeletal muscle. [provided by RefSeq, Jul 2008]
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UniProt Comments for MYOG

myogenin: Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: nucleoplasm; nucleus; transcription factor complex

Molecular Function: chromatin DNA binding; DNA binding transcription factor activity; protein binding; protein heterodimerization activity; sequence-specific DNA binding; transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding

Biological Process: cell cycle; cellular response to estradiol stimulus; cellular response to growth factor stimulus; cellular response to lithium ion; cellular response to retinoic acid; cellular response to tumor necrosis factor; mRNA transcription from RNA polymerase II promoter; muscle cell fate commitment; myoblast differentiation; negative regulation of cell proliferation; negative regulation of glycolytic process; ossification; positive regulation of cell cycle arrest; positive regulation of muscle atrophy; positive regulation of muscle cell differentiation; positive regulation of myoblast differentiation; positive regulation of myotube differentiation; positive regulation of oxidative phosphorylation; positive regulation of skeletal muscle fiber development; positive regulation of transcription from RNA polymerase II promoter; regulation of skeletal muscle satellite cell proliferation; response to denervation involved in regulation of muscle adaptation; response to electrical stimulus involved in regulation of muscle adaptation; response to gravity; response to muscle activity involved in regulation of muscle adaptation; skeletal muscle atrophy; skeletal muscle fiber development; skeletal muscle tissue development; skeletal muscle tissue regeneration; striated muscle atrophy
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Research Articles on MYOG

1. Findings indicate a interplay between A-kinase anchoring protein 6 (AKAP6) and myogenin.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with MYOG elisa kit

 Reference Product  PubMed Publications
 MEF2A elisa kit  >14 publications with MYOG and MEF2A
 MEF2C elisa kit  >13 publications with MYOG and MEF2C
 SIX1 elisa kit  >10 publications with MYOG and SIX1
 SRF elisa kit  >8 publications with MYOG and SRF
 TCF3 elisa kit  >2 publications with MYOG and TCF3
 PRMT5 elisa kit  >1 publications with MYOG and PRMT5
 POLR2C elisa kit  >1 publications with MYOG and POLR2C
 CSRP3 elisa kit  >1 publications with MYOG and CSRP3

Pathways associated with MYOG elisa kit

 Products by Pathway  Pathway Diagram
 CDO In Myogenesis Pathway antibodies  CDO In Myogenesis Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Id Signaling Pathway antibodies  Id Signaling Pathway Diagram
 Myogenesis Pathway antibodies  Myogenesis Pathway Diagram
 Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway antibodies  Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway Diagram

Diseases associated with MYOG elisa kit

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >58 publications with MYOG and Neoplasms
 Nervous System Diseases Antibodies  >49 publications with MYOG and Nervous System Diseases
 Necrosis Antibodies  >19 publications with MYOG and Necrosis
 Inflammation Antibodies  >12 publications with MYOG and Inflammation
 Fibrosis Antibodies  >12 publications with MYOG and Fibrosis
 Insulin Resistance Antibodies  >9 publications with MYOG and Insulin Resistance
 Cell Transformation, Neoplastic Antibodies  >9 publications with MYOG and Cell Transformation, Neoplastic
 Kidney Diseases Antibodies  >7 publications with MYOG and Kidney Diseases
 Hyperplasia Antibodies  >7 publications with MYOG and Hyperplasia
 Chromosome Aberrations Antibodies  >6 publications with MYOG and Chromosome Aberrations

Organs/Tissues associated with MYOG elisa kit

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >729 publications with MYOG and Muscle
 Nerve Antibodies  >52 publications with MYOG and Nerve
 Eye Antibodies  >8 publications with MYOG and Eye
 Placenta Antibodies  >2 publications with MYOG and Placenta
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