Bovine FAM175A ELISA Kit – Cat# MBS285679

BRCA1-A Complex Subunit Abraxas (FAM175A) ELISA Kit for Bovine

Name: Bovine FAM175A ELISA Kit; BRCA1-A Complex Subunit Abraxas; MyBioSource
Brand: MyBioSource
SKU: MBS285679
Price: 545 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS285679

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate FAM175A in samples. An antibody specific for FAM175A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFAM175A present is bound by… More details

$545 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

BRCA1-A complex subunit Abraxas (FAM175A), ELISA Kit

Full Product Name

Bovine BRCA1-A complex subunit Abraxas (FAM175A) ELISA Kit

Product Gene Name

FAM175A elisa kit

Product Synonym Names

ABRA1; CCDC98; FLJ11520; FLJ12642; FLJ13614; abraxas protein|coiled-coil domain containing 98

Species Reactivity

Bovine

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Bovine FAM175A. No significant cross-reactivity or interference between Bovine FAM175A and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

46,184 Da

3D Structure

ModBase 3D Structure for Q5E9P1

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of FAM175A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FAM175A purchase

MBS285679 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the BRCA1-A complex subunit Abraxas (FAM175A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FAM175A. The ELISA analytical biochemical technique of the MBS285679 kit is based on FAM175A antibody-FAM175A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FAM175A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FAM175A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for FAM175A. It may not necessarily be applicable to this product.

NCBI GI #

62751618

NCBI GeneID

504796

NCBI Accession #

NP_001015516.1

UniProt Primary Accession #

Q5E9P1

UniProt Secondary Accession #

A4FV82

UniProt Related Accession #

Q5E9P1

GenBank Nucleotide #

NM_001015516.1

NCBI Official Full Name

BRCA1-A complex subunit Abraxas 1

NCBI Official Symbol

ABRAXAS1

NCBI Official Synonym Symbols

CCDC98; FAM175A

UniProt Protein Name

BRCA1-A complex subunit Abraxas 1

UniProt Synonym Protein Names

Coiled-coil domain-containing protein 98; Protein FAM175A

Protein Family

BRCA1-A complex

UniProt Gene Name

ABRAXAS1

NCBI Protein Information

BRCA1-A complex subunit Abraxas 1; BRCA1-A complex subunit Abraxas

UniProt Comments for FAM175A

Involved in DNA damage response and double-strand break (DSB) repair. Component of the BRCA1-A complex, acting as a central scaffold protein that assembles the various components of the complex and mediates the recruitment of BRCA1. The BRCA1-A complex specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesion sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at DSBs. This complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with FAM175A elisa kit

Disease Name	Pubmed Publications
Breast Neoplasms Antibodies	>5 publications with FAM175A and Breast Neoplasms
Ovarian Neoplasms Antibodies	>3 publications with FAM175A and Ovarian Neoplasms
Carcinoma, Squamous Cell Antibodies	>1 publications with FAM175A and Carcinoma, Squamous Cell
Intestinal Diseases Antibodies	>1 publications with FAM175A and Intestinal Diseases