Matrix Metalloproteinase 14 (MMP14) ELISA Kit for Human

Background: matrix metallopeptidase 14 (MMP14) Homo sapiens Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MMP14 in Human serum, plasma. An antibody specific for MMP14 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP14 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP14 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP14 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Intended Uses: For the quantitative detection of Human Matrix Metalloproteinase 14 (MMP14) concentration in serum, plasma and other biological fluids.

Small volumes of MMP14 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS285685 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Matrix Metalloproteinase 14 (MMP14) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MMP14. The ELISA analytical biochemical technique of the MBS285685 kit is based on MMP14 antibody-MMP14 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MMP14 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MMP14. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).