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Acetylcholinesterase (ACHE) ELISA Kit for Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS285759

Intended Uses: For the quantitative detection of Mouse Acetylcholinesterase (ACHE) concentration in serum, plasma and other biological fluids.Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ACHE in Mouse serum, plasma. An… More details

$545 / 48 Tests +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Acetylcholinesterase (ACHE), ELISA Kit
Full Product Name
Mouse Acetylcholinesterase (ACHE) ELISA Kit
Product Gene Name
ACHE elisa kit
Product Synonym Names
ARACHE; N-ACHE; YT; OTTHUMP00000211347|OTTHUMP00000211349|OTTHUMP00000211356|acetylcholinesterase|apoptosis-related acetylcholinesterase
Species Reactivity
Mouse
Assay Type
Quantitative Sandwich
Samples
Serum, plasma and other biological fluids
Detection Range
0.31 mU/mL - 20 mU/mL
Sensitivity
0.156 mU/mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse ACHE. No significant cross-reactivity or interference between Mouse ACHE and analogues was observed.
Preparation and Storage
Store at 2-8 degree C.
Inter-assay Precision
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
Intra-assay Precision
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Molecular Weight
– Da
3D Structure
ModBase 3D Structure for P21836
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of ACHE elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ACHE purchase

MBS285759 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Acetylcholinesterase (ACHE) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ACHE. The ELISA analytical biochemical technique of the MBS285759 kit is based on ACHE antibody-ACHE antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ACHE antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ACHE. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for ACHE. It may not necessarily be applicable to this product.

NCBI GI #
586597902
NCBI GeneID
11423
NCBI Accession #
NP_001276939.1
UniProt Primary Accession #
P21836
UniProt Related Accession #
P21836
GenBank Nucleotide #
NM_001290010.1
NCBI Official Full Name
acetylcholinesterase
NCBI Official Synonym Full Names
acetylcholinesterase
NCBI Official Symbol
Ache
UniProt Protein Name
Acetylcholinesterase
Protein Family
Acetylcholinesterase
UniProt Gene Name
Ache
UniProt Synonym Gene Names
AChE
NCBI Protein Information
acetylcholinesterase

UniProt Comments for ACHE

AChE: Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis. Belongs to the type-B carboxylesterase/lipase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.1.7; Hydrolase; Lipid Metabolism - glycerophospholipid; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 5 G2|5 76.32 cM

Cellular Component: axon; basal lamina; cell junction; cell soma; cell surface; cytoplasm; dendrite; endoplasmic reticulum lumen; extracellular region; extracellular space; Golgi apparatus; membrane; neuromuscular junction; neuron projection; nuclear envelope; perinuclear region of cytoplasm; plasma membrane; postsynaptic membrane; presynaptic membrane; rough endoplasmic reticulum; synapse

Molecular Function: acetylcholine binding; acetylcholinesterase activity; choline binding; cholinesterase activity; collagen binding; hydrolase activity; laminin binding; protein binding; protein homodimerization activity; protein self-association; serine hydrolase activity

Biological Process: acetylcholine catabolic process; acetylcholine metabolic process; cell adhesion; choline metabolic process; neurotransmitter catabolic process; neurotransmitter receptor biosynthetic process; positive regulation of axonogenesis; positive regulation of dendrite morphogenesis; protein tetramerization; receptor internalization; regulation of receptor recycling; retina development in camera-type eye; synaptogenesis
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Research Articles on ACHE

1. Results indicate that direct inhibition of acetylcholinesterase activity and up-regulation of m1 muscarinic acetylcholine receptor expression in the striatum might contribute to the beneficial effects of alpha-asarone on locomotor hyperactivity in Fmr1 knock out mice.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ACHE elisa kit

 Reference Product  PubMed Publications
 Chat elisa kit  >734 publications with ACHE and Chat
 Colq elisa kit  >94 publications with ACHE and Colq
 Slc44a1 elisa kit  >1 publications with ACHE and Slc44a1

Pathways associated with ACHE elisa kit

 Products by Pathway  Pathway Diagram
 Acetylcholine Synthesis Pathway antibodies  Acetylcholine Synthesis Pathway Diagram
 Biogenic Amine Synthesis Pathway antibodies  Biogenic Amine Synthesis Pathway Diagram
 Cholinergic Synapse Pathway antibodies  Cholinergic Synapse Pathway Diagram
 Glycerophospholipid Biosynthesis Pathway antibodies  Glycerophospholipid Biosynthesis Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Neuronal System Pathway antibodies  Neuronal System Pathway Diagram
 Neurotransmitter Clearance In The Synaptic Cleft Pathway antibodies  Neurotransmitter Clearance In The Synaptic Cleft Pathway Diagram

Diseases associated with ACHE elisa kit

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >372379 publications with ACHE and Nervous System Diseases
 Peripheral Nervous System Diseases Antibodies  >34247 publications with ACHE and Peripheral Nervous System Diseases
 Brain Diseases Antibodies  >34141 publications with ACHE and Brain Diseases
 Inflammation Antibodies  >27676 publications with ACHE and Inflammation
 Necrosis Antibodies  >14256 publications with ACHE and Necrosis
 Hypertension Antibodies  >12747 publications with ACHE and Hypertension
 Edema Antibodies  >12352 publications with ACHE and Edema
 Kidney Diseases Antibodies  >11340 publications with ACHE and Kidney Diseases
 Disease Models, Animal Antibodies  >10374 publications with ACHE and Disease Models, Animal
 Liver Diseases Antibodies  >9859 publications with ACHE and Liver Diseases

Organs/Tissues associated with ACHE elisa kit

 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >43533 publications with ACHE and Heart
 Brain Antibodies  >41945 publications with ACHE and Brain
 Spinal Cord Antibodies  >25438 publications with ACHE and Spinal Cord
 Liver Antibodies  >15885 publications with ACHE and Liver
 Intestine Antibodies  >13102 publications with ACHE and Intestine
 Eye Antibodies  >8995 publications with ACHE and Eye
 Spleen Antibodies  >2432 publications with ACHE and Spleen
 Embryonic Tissue Antibodies  >874 publications with ACHE and Embryonic Tissue
 Thymus Antibodies  >471 publications with ACHE and Thymus
 Inner Ear Antibodies  >304 publications with ACHE and Inner Ear
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