Dyslexia Susceptibility 1 Candidate Gene (DYX1C1) ELISA Kit for Human

DYX1C1 encodes a tetratricopeptide repeat domain-containing protein. The encoded protein interacts with estrogen receptors and the heat shock proteins, Hsp70 and Hsp90. A chromosomal translocation involving this gene is associated with a susceptibility to developmental dyslexia. Mutations in this gene are associated with deficits in reading and spelling. Alternate splicing results in multiple transcript variants.
The DYX1C1 gene encodes a 420-amino acid protein with 3 tetratricopeptide repeat (TPR) domains, thought to be protein interaction modules. Mouse Dyx1c1 protein is 78% identical to the human protein, and nonhuman primates differ at 0.5-1.4% of residues.In human brain, DYX1C1 protein localizes to a fraction of cortical neurons and white matter glial cells.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate DYX1C1 in samples. An antibody specific for DYX1C1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDYX1C1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DYX1C1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DYX1C1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of DYX1C1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS285772 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Dyslexia susceptibility 1 candidate gene (DYX1C1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DYX1C1. The ELISA analytical biochemical technique of the MBS285772 kit is based on DYX1C1 antibody-DYX1C1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DYX1C1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DYX1C1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).