Procollagen C Peptidase (PCP) ELISA Kit for Human

Name: Human PCP ELISA Kit; Procollagen C Peptidase; MyBioSource
Brand: MyBioSource
SKU: MBS285804
Price: 545 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS285804

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PCP in samples. An antibody specific for PCP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPCP present is bound by the… More details

$545 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Procollagen C peptidase (PCP), ELISA Kit

Full Product Name

Human Procollagen C peptidase (PCP) ELISA Kit

Product Gene Name

PCP elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human PCP. No significant cross-reactivity or interference between Human PCP and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

92,655 Da

OMIM

112264

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of PCP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PCP purchase

MBS285804 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Procollagen C peptidase (PCP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PCP. The ELISA analytical biochemical technique of the MBS285804 kit is based on PCP antibody-PCP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PCP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PCP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PCP. It may not necessarily be applicable to this product.

NCBI GI #

1245357

NCBI GeneID

649

NCBI Accession #

AAA93462.1

UniProt Secondary Accession #

Q13292; Q13872; Q14874; Q99421; Q99422; Q99423; Q9UL38; A8K6F5; B2RN46; D3DSR0

UniProt Related Accession #

P13497

NCBI Official Full Name

procollagen C-proteinase

NCBI Official Synonym Full Names

bone morphogenetic protein 1

NCBI Official Symbol

BMP1

NCBI Official Synonym Symbols

PCP; TLD; OI13; PCP2; PCOLC

UniProt Protein Name

Bone morphogenetic protein 1

UniProt Synonym Protein Names

Mammalian tolloid protein; mTld; Procollagen C-proteinase; PCP

Protein Family

Outer membrane lipoprotein

UniProt Gene Name

BMP1

UniProt Synonym Gene Names

PCOLC; BMP-1; mTld; PCP

UniProt Entry Name

BMP1_HUMAN

NCBI Protein Information

bone morphogenetic protein 1

NCBI Summary for PCP

This gene encodes a protein that is capable of inducing formation of cartilage in vivo. Although other bone morphogenetic proteins are members of the TGF-beta superfamily, this gene encodes a protein that is not closely related to other known growth factors. This gene is expressed as alternatively spliced variants that share an N-terminal protease domain but differ in their C-terminal region. [provided by RefSeq, Aug 2008]

UniProt Comments for PCP

BMP1: Cleaves the C-terminal propeptides of procollagen I, II and III. Induces cartilage and bone formation. May participate in dorsoventral patterning during early development by cleaving chordin (CHRD). Defects in BMP1 are a cause of autosomal recessive osteogenesis imperfecta (AR-OI). A connective tissue disorder characterized by bone fragility, progressively deforming bones, bowing of limbs due to multiple fractures, very short stature, a triangular face, severe scoliosis, and grayish sclera. AR-OI due to BMP1 mutations belongs to the group of osteogenesis imperfecta type III in the Sillence classification. Belongs to the peptidase M12A family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.24.19; Cytokine; Protease

Chromosomal Location of Human Ortholog: 8p21.3

Cellular Component: extracellular region; extracellular space; Golgi apparatus; proteinaceous extracellular matrix

Molecular Function: calcium ion binding; cytokine activity; growth factor activity; metalloendopeptidase activity; metallopeptidase activity; peptidase activity; protein binding; transforming growth factor beta receptor binding; zinc ion binding

Biological Process: BMP signaling pathway; cartilage condensation; cell development; extracellular matrix disassembly; extracellular matrix organization and biogenesis; lipoprotein metabolic process; multicellular organismal development; ossification; proteolysis; regulation of apoptosis; regulation of MAPKKK cascade; skeletal development

Disease: Osteogenesis Imperfecta, Type Xiii

Research Articles on PCP

1. Two novel variants in the BMP1 gene: c.808A>G and c.1297G>T care associated with osteogenesis imperfecta.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PCP elisa kit

Reference Product	PubMed Publications
LOX elisa kit	>8 publications with PCP and LOX
PRL elisa kit	>2 publications with PCP and PRL
ADAMTS2 elisa kit	>1 publications with PCP and ADAMTS2
COL7A1 elisa kit	>1 publications with PCP and COL7A1
LAMC2 elisa kit	>1 publications with PCP and LAMC2
CHRD elisa kit	>1 publications with PCP and CHRD

Pathways associated with PCP elisa kit

Products by Pathway	Pathway Diagram
Adipogenesis Pathway antibodies	Adipogenesis Pathway Diagram
Anchoring Fibril Formation Pathway antibodies	Anchoring Fibril Formation Pathway Diagram
Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway antibodies	Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway Diagram
Cardiac Progenitor Differentiation Pathway antibodies	Cardiac Progenitor Differentiation Pathway Diagram
Collagen Biosynthesis And Modifying Enzymes Pathway antibodies	Collagen Biosynthesis And Modifying Enzymes Pathway Diagram
Collagen Formation Pathway antibodies	Collagen Formation Pathway Diagram
Crosslinking Of Collagen Fibrils Pathway antibodies	Crosslinking Of Collagen Fibrils Pathway Diagram
Degradation Of The Extracellular Matrix Pathway antibodies	Degradation Of The Extracellular Matrix Pathway Diagram
Extracellular Matrix Organization Pathway antibodies	Extracellular Matrix Organization Pathway Diagram
HDL-mediated Lipid Transport Pathway antibodies	HDL-mediated Lipid Transport Pathway Diagram

Diseases associated with PCP elisa kit

Disease Name	Pubmed Publications
Fibrosis Antibodies	>24 publications with PCP and Fibrosis
Nervous System Diseases Antibodies	>10 publications with PCP and Nervous System Diseases
Neoplasm Metastasis Antibodies	>6 publications with PCP and Neoplasm Metastasis
Inflammation Antibodies	>6 publications with PCP and Inflammation
Breast Neoplasms Antibodies	>5 publications with PCP and Breast Neoplasms
Kidney Diseases Antibodies	>5 publications with PCP and Kidney Diseases
Myocardial Infarction Antibodies	>5 publications with PCP and Myocardial Infarction
Necrosis Antibodies	>5 publications with PCP and Necrosis
Lung Neoplasms Antibodies	>4 publications with PCP and Lung Neoplasms
Neoplasms, Experimental Antibodies	>3 publications with PCP and Neoplasms, Experimental

Organs/Tissues associated with PCP elisa kit

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>255 publications with PCP and Bone
Connective Tissue Antibodies	>41 publications with PCP and Connective Tissue
Embryonic Tissue Antibodies	>28 publications with PCP and Embryonic Tissue
Muscle Antibodies	>23 publications with PCP and Muscle
Vascular Antibodies	>17 publications with PCP and Vascular
Kidney Antibodies	>17 publications with PCP and Kidney
Heart Antibodies	>17 publications with PCP and Heart
Bone Marrow Antibodies	>16 publications with PCP and Bone Marrow
Skin Antibodies	>15 publications with PCP and Skin
Eye Antibodies	>14 publications with PCP and Eye