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COUP Transcription Factor 2 (NR2F2) ELISA Kit for Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS285810

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate NR2F2 in samples. An antibody specific for NR2F2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNR2F2 present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
COUP transcription factor 2 (NR2F2), ELISA Kit
Full Product Name
Mouse COUP transcription factor 2 (NR2F2) ELISA Kit
Product Gene Name
NR2F2 elisa kit
Product Synonym Names
ARP1; COUP-TFII; COUPTFB; MGC117452; SVP40; TFCOUP2; ADP-ribosylation factor related protein 1|ovalbumin upstream promoter transcription factor II|transcription factor COUP 2 (chicken ovalbumin upst
Species Reactivity
Mouse
Assay Type
Sandwich
Samples
Serum, Plasma, Other biological fluids
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse NR2F2. No significant cross-reactivity or interference between Mouse NR2F2 and analogues was observed.
Preparation and Storage
Store at 2-8 degree C.
Molecular Weight
45,571 Da
3D Structure
ModBase 3D Structure for P43135
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of NR2F2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NR2F2 purchase

MBS285810 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the COUP transcription factor 2 (NR2F2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NR2F2. The ELISA analytical biochemical technique of the MBS285810 kit is based on NR2F2 antibody-NR2F2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NR2F2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NR2F2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for NR2F2. It may not necessarily be applicable to this product.

NCBI GI #
45598394
NCBI GeneID
11819
NCBI Accession #
NP_033827.2
UniProt Primary Accession #
P43135
UniProt Related Accession #
P43135
GenBank Nucleotide #
NM_009697.3
NCBI Official Full Name
COUP transcription factor 2 isoform 1
NCBI Official Synonym Full Names
nuclear receptor subfamily 2, group F, member 2
NCBI Official Symbol
Nr2f2
NCBI Official Synonym Symbols
EAR3; ARP-1; SVP40; Aporp1; COUPTFB; COUP-TF2; Tcfcoup2; COUP-TFII; 2700033K02Rik; 9430015G03Rik
UniProt Protein Name
COUP transcription factor 2
UniProt Synonym Protein Names
Apolipoprotein AI regulatory protein 1; ARP-1; COUP transcription factor II; COUP-TF II; Nuclear receptor subfamily 2 group F member 2
Protein Family
COUP transcription factor
UniProt Gene Name
Nr2f2
UniProt Synonym Gene Names
Aporp1; Arp-1; Arp1; Tfcoup2; COUP-TF2; ARP-1; COUP-TF II
NCBI Protein Information
COUP transcription factor 2

UniProt Comments for NR2F2

NR2F2: Ligand-activated transcription factor. Activated by high concentrations of 9-cis-retinoic acid and all-trans-retinoic acid, but not by dexamethasone, cortisol or progesterone (in vitro). Regulation of the apolipoprotein A-I gene transcription. Binds to DNA site A. Interacts with SQSTM1. Binds DNA as a dimer; homodimer or heterodimer with NR2F6. Interacts with NCOA1, NCOA2, NCOA3 and PPARGC1A. Interacts with ZFPM2. Ubiquitous. Belongs to the nuclear hormone receptor family. NR2 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Motility/polarity/chemotaxis; Nuclear receptor

Chromosomal Location of Human Ortholog: 7 C|7 38.66 cM

Cellular Component: nucleus

Molecular Function: DNA binding; DNA binding transcription factor activity; ligand-dependent nuclear receptor activity; metal ion binding; protein homodimerization activity; retinoic acid binding; sequence-specific DNA binding; steroid hormone receptor activity; zinc ion binding

Biological Process: anterior/posterior pattern specification; blood vessel morphogenesis; fertilization; forebrain development; in utero embryonic development; limb development; lymph vessel development; maternal placenta development; negative regulation of cyclin-dependent protein serine/threonine kinase activity; negative regulation of endothelial cell proliferation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; neuron migration; positive regulation of systemic arterial blood pressure; positive regulation of transcription, DNA-templated; radial pattern formation; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-templated; skeletal muscle tissue development; steroid hormone mediated signaling; transcription, DNA-dependent
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Research Articles on NR2F2

1. elimination of Wolffian ducts in female embryos is actively promoted by COUP-TFII, which suppresses a mesenchyme-epithelium cross-talk responsible for Wolffian duct maintenance.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with NR2F2 elisa kit

 Reference Product  PubMed Publications
 Dr1 elisa kit  >5 publications with NR2F2 and Dr1
 Pax6 elisa kit  >4 publications with NR2F2 and Pax6
 Sox9 elisa kit  >3 publications with NR2F2 and Sox9
 Nrp1 elisa kit  >2 publications with NR2F2 and Nrp1
 Runx2 elisa kit  >2 publications with NR2F2 and Runx2
 Rere elisa kit  >1 publications with NR2F2 and Rere
 Zfpm2 elisa kit  >1 publications with NR2F2 and Zfpm2
 Pck1 elisa kit  >1 publications with NR2F2 and Pck1

Pathways associated with NR2F2 elisa kit

 Products by Pathway  Pathway Diagram
 Nuclear Receptors Pathway antibodies  Nuclear Receptors Pathway Diagram
 PluriNetWork Pathway antibodies  PluriNetWork Pathway Diagram
 PodNet: Protein-protein Interactions In The Podocyte Pathway antibodies  PodNet: Protein-protein Interactions In The Podocyte Pathway Diagram
 XPodNet - Protein-protein Interactions In The Podocyte Expanded By STRING Pathway antibodies  XPodNet - Protein-protein Interactions In The Podocyte Expanded By STRING Pathway Diagram

Diseases associated with NR2F2 elisa kit

 Disease Name  Pubmed Publications
 Adenocarcinoma Antibodies  >10 publications with NR2F2 and Adenocarcinoma
 Heart Diseases Antibodies  >7 publications with NR2F2 and Heart Diseases
 Neoplasm Metastasis Antibodies  >6 publications with NR2F2 and Neoplasm Metastasis
 Breast Neoplasms Antibodies  >6 publications with NR2F2 and Breast Neoplasms
 Cell Transformation, Neoplastic Antibodies  >6 publications with NR2F2 and Cell Transformation, Neoplastic
 Hernia, Diaphragmatic Antibodies  >6 publications with NR2F2 and Hernia, Diaphragmatic
 Carcinoma, Hepatocellular Antibodies  >5 publications with NR2F2 and Carcinoma, Hepatocellular
 Liver Neoplasms Antibodies  >5 publications with NR2F2 and Liver Neoplasms
 Liver Diseases Antibodies  >4 publications with NR2F2 and Liver Diseases
 Infertility, Male Antibodies  >3 publications with NR2F2 and Infertility, Male

Organs/Tissues associated with NR2F2 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >37 publications with NR2F2 and Liver
 Brain Antibodies  >22 publications with NR2F2 and Brain
 Embryonic Tissue Antibodies  >17 publications with NR2F2 and Embryonic Tissue
 Heart Antibodies  >15 publications with NR2F2 and Heart
 Lung Antibodies  >11 publications with NR2F2 and Lung
 Kidney Antibodies  >8 publications with NR2F2 and Kidney
 Uterus Antibodies  >7 publications with NR2F2 and Uterus
 Eye Antibodies  >6 publications with NR2F2 and Eye
 Intestine Antibodies  >6 publications with NR2F2 and Intestine
 Testis Antibodies  >5 publications with NR2F2 and Testis
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