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S-Phase Kinase-Associated Protein 1 (SKP1) ELISA Kit for Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS285812

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate SKP1 in samples. An antibody specific for SKP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySKP1 present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
S-phase kinase-associated protein 1 (SKP1), ELISA Kit
Full Product Name
Mouse S-phase kinase-associated protein 1 (SKP1) ELISA Kit
Product Gene Name
SKP1 elisa kit
Product Synonym Names
EMC19; MGC34403; OCP-II; OCP2; SKP1A; TCEB1L; p19A; RNA polymerase II elongation factor-like protein OCP2|cyclin A/CDK2-associated p19|organ of Corti protein 2|transcription elongation factor B (SII
Species Reactivity
Mouse
Assay Type
Sandwich
Samples
Serum, Plasma, Other biological fluids
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse SKP1. No significant cross-reactivity or interference between Mouse SKP1 and analogues was observed.
Preparation and Storage
Store at 2-8 degree C.
Molecular Weight
18,672 Da
3D Structure
ModBase 3D Structure for Q9WTX5
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of SKP1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SKP1 purchase

MBS285812 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the S-phase kinase-associated protein 1 (SKP1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SKP1. The ELISA analytical biochemical technique of the MBS285812 kit is based on SKP1 antibody-SKP1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SKP1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SKP1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for SKP1. It may not necessarily be applicable to this product.

NCBI GI #
158854016
NCBI GeneID
21402
NCBI Accession #
NP_035673.3
UniProt Primary Accession #
Q9WTX5
UniProt Secondary Accession #
Q8C5H6
UniProt Related Accession #
Q9WTX5
GenBank Nucleotide #
NM_011543.4
NCBI Official Full Name
S-phase kinase-associated protein 1
NCBI Official Synonym Full Names
S-phase kinase-associated protein 1A
NCBI Official Symbol
Skp1a
NCBI Official Synonym Symbols
OCP2; SKP1; p19A; 15kDa; EMC19; OCP-II; Tceb1l; p19Skp1; 2610043E24Rik; 2610206H23Rik
UniProt Protein Name
S-phase kinase-associated protein 1
UniProt Synonym Protein Names
Cyclin-A/CDK2-associated protein p19; S-phase kinase-associated protein 1A; p19A; p19skp1
Protein Family
S-phase kinase-associated protein
UniProt Gene Name
Skp1
UniProt Synonym Gene Names
Skp1a
NCBI Protein Information
S-phase kinase-associated protein 1

UniProt Comments for SKP1

SKP1A: Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(Cyclin F) directs ubiquitination of CP110. Component of an E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X. Component of the SCF(BTRC) complex, composed of SKP1, CUL1 and BTRC. Part of a SCF(BTRC)-like complex lacking CUL1, which is associated with phosphorylated NFKBIA and RELA; RELA interacts directly with NFKBIA. Component of the SCF(FBXO44) complex, composed of SKP1, CUL1 and FBXO44. Identified in a complex with SKP2, CKS1B and p27Kip1. Interacts with the cyclin A/CDK2 complex. Part of a SCF- like complex consisting of CUL7, RBX1, SKP1 and FBXW8. Part of several SCF complexes containing SKP1 and one of the F-box proteins. Component of a SCF(SKP2)-like complex containing CUL1, SKP1, TRIM21 and SKP2. Interacts with FBXO2, FBXO4, FBXW7 and TRIM21. Interacts with FBXO45. Component of the SCF(FBXO17) complex, composed of SKP1, CUL1 and FBXO17. Component of the SCF(FBXO27) complex, composed of SKP1, CUL1 and FBXO27. Component of the SCF(Cyclin F) complex consisting of CUL1, RBX1, SKP1 and CCNF. Belongs to the SKP1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 11 B1.3|11 31.86 cM

Cellular Component: centrosome; cytoplasm; cytosol; nucleus; PcG protein complex; SCF ubiquitin ligase complex

Molecular Function: beta-catenin binding; protein binding; protein domain specific binding; ubiquitin-protein transferase activity

Biological Process: maintenance of protein location in nucleus; protein ubiquitination; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; ubiquitin-dependent protein catabolic process
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Research Articles on SKP1

1. Consistent with Skp1a functioning through regulation of Nmnat2, Skp1a knockdown fails to protect axons from Nmnat2 knockdown.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with SKP1 elisa kit

 Products by Pathway  Pathway Diagram
 APC/C-mediated Degradation Of Cell Cycle Proteins Pathway antibodies  APC/C-mediated Degradation Of Cell Cycle Proteins Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Circadian Rhythm - Mammal Pathway antibodies  Circadian Rhythm - Mammal Pathway Diagram
 Circadian Rhythm - Mammal Pathway antibodies  Circadian Rhythm - Mammal Pathway Diagram
 Cyclin A:Cdk2-associated Events At S Phase Entry Pathway antibodies  Cyclin A:Cdk2-associated Events At S Phase Entry Pathway Diagram
 Cyclin E Associated Events During G1/S Transition Pathway antibodies  Cyclin E Associated Events During G1/S Transition Pathway Diagram
 Degradation Of Beta-catenin By The Destruction Complex Pathway antibodies  Degradation Of Beta-catenin By The Destruction Complex Pathway Diagram
 Delta-Notch Signaling Pathway antibodies  Delta-Notch Signaling Pathway Diagram

Diseases associated with SKP1 elisa kit

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >5 publications with SKP1 and Nervous System Diseases
 Disease Models, Animal Antibodies  >2 publications with SKP1 and Disease Models, Animal
 Inflammation Antibodies  >2 publications with SKP1 and Inflammation
 Neoplasms Antibodies  >2 publications with SKP1 and Neoplasms
 Carcinoma Antibodies  >1 publications with SKP1 and Carcinoma
 Adenoma, Liver Cell Antibodies  >1 publications with SKP1 and Adenoma, Liver Cell
 Hyperplasia Antibodies  >1 publications with SKP1 and Hyperplasia

Organs/Tissues associated with SKP1 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >3 publications with SKP1 and Brain
 Adrenal Gland Antibodies  >1 publications with SKP1 and Adrenal Gland
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