Xenotropic and Polytropic Retrovirus Receptor 1 (XPR1) ELISA Kit for Human

Expression of XPR1 in mouse and hamster MLV-resistant fibroblasts rendered the cells susceptible to both X- and P-MLV. The deduced 696-amino acid XPR1 protein contains 8 or 9 potential membrane-spanning regions, 7 potential N-glycosylation sites, and 7 dileucines that may stimulate endocytosis via clathrin-coated pits.
Northern blot analysis detected a 4.5-kb XPR1 transcript in all tissues tested, with highest expression in pancreas, kidney, placenta, hematopoietic tissues, and heart, and lowest expression in skeletal muscle. Expression of XPR1 was greater in fetal liver than adult liver. A 9.5-kb XPR1 transcript was also detected in all tissues tested except liver and bone marrow.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate XPR1 in samples. An antibody specific for XPR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyXPR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for XPR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of XPR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of XPR1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS285826 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Xenotropic and polytropic retrovirus receptor 1 (XPR1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing XPR1. The ELISA analytical biochemical technique of the MBS285826 kit is based on XPR1 antibody-XPR1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect XPR1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, XPR1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).