Prostaglandin E2 (PG-E2) ELISA Kit for Human

Name: Human PG-E2 ELISA Kit; Prostaglandin E2; MyBioSource
Brand: MyBioSource
SKU: MBS285915
Price: 460 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS285915

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PG-E2 in samples. An antibody specific for PG-E2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPG-E2 present is bound by the… More details

$460 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Prostaglandin E2 (PG-E2), ELISA Kit

Full Product Name

Human Prostaglandin E2 (PG-E2) ELISA Kit

Product Gene Name

PG-E2 elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human PG-E2. No significant cross-reactivity or interference between Human PG-E2 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

53,119 Da

OMIM

601586

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of PG-E2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PG-E2 purchase

MBS285915 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Prostaglandin E2 (PG-E2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PG-E2. The ELISA analytical biochemical technique of the MBS285915 kit is based on PG-E2 antibody-PG-E2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PG-E2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PG-E2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PG-E2. It may not necessarily be applicable to this product.

NCBI GI #

4506259

NCBI GeneID

5734

NCBI Accession #

NP_000949.1

UniProt Secondary Accession #

Q3MJ87

UniProt Related Accession #

P35408

GenBank Nucleotide #

NM_000958.2

NCBI Official Full Name

prostaglandin E2 receptor EP4 subtype

NCBI Official Synonym Full Names

prostaglandin E receptor 4

NCBI Official Symbol

PTGER4

NCBI Official Synonym Symbols

EP4; EP4R

UniProt Protein Name

Prostaglandin E2 receptor EP4 subtype

UniProt Synonym Protein Names

Prostanoid EP4 receptor

Protein Family

Prostaglandin E2 receptor

UniProt Gene Name

PTGER4

UniProt Synonym Gene Names

PTGER2; PGE receptor EP4 subtype; PGE2 receptor EP4 subtype

UniProt Entry Name

PE2R4_HUMAN

NCBI Protein Information

prostaglandin E2 receptor EP4 subtype

NCBI Summary for PG-E2

The protein encoded by this gene is a member of the G-protein coupled receptor family. This protein is one of four receptors identified for prostaglandin E2 (PGE2). This receptor can activate T-cell factor signaling. It has been shown to mediate PGE2 induced expression of early growth response 1 (EGR1), regulate the level and stability of cyclooxygenase-2 mRNA, and lead to the phosphorylation of glycogen synthase kinase-3. Knockout studies in mice suggest that this receptor may be involved in the neonatal adaptation of circulatory system, osteoporosis, as well as initiation of skin immune responses. [provided by RefSeq, Jul 2008]

UniProt Comments for PG-E2

PTGER4: Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 5p13.1

Cellular Component: cell soma; integral to membrane; nuclear membrane; plasma membrane

Molecular Function: prostaglandin E receptor activity; protein binding

Biological Process: chemokinesis; female pregnancy; G-protein signaling, coupled to cAMP nucleotide second messenger; immune response; JNK cascade; maternal process involved in parturition; negative regulation of circadian sleep/wake cycle, REM sleep; negative regulation of cytokine secretion; negative regulation of inflammatory response; negative regulation of integrin activation; negative regulation of interleukin-1 alpha secretion; negative regulation of nitric-oxide synthase biosynthetic process; negative regulation of peptide secretion; positive regulation of bone resorption; positive regulation of cAMP biosynthetic process; positive regulation of cell adhesion; positive regulation of cell proliferation; positive regulation of circadian sleep/wake cycle, non-REM sleep; positive regulation of cytokine secretion; positive regulation of inflammatory response; positive regulation of interleukin-8 production; positive regulation of osteoblast differentiation; positive regulation of smooth muscle cell migration; positive regulation of tyrosine phosphorylation of Stat3 protein; regulation of stress fiber formation; response to amino acid stimulus; response to drug; response to lipopolysaccharide; response to mechanical stimulus; response to progesterone stimulus; T-helper cell differentiation

Research Articles on PG-E2

1. Altered inhibitory function of the EP4R in eosinophils and monocytes from aspirin-intolerant patients

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PG-E2 elisa kit

Reference Product	PubMed Publications
PTGS2 elisa kit	>153 publications with PG-E2 and PTGS2
SRC elisa kit	>8 publications with PG-E2 and SRC
FEM1A elisa kit	>1 publications with PG-E2 and FEM1A
PTHLH elisa kit	>1 publications with PG-E2 and PTHLH

Pathways associated with PG-E2 elisa kit

Products by Pathway	Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
Eicosanoid Ligand-binding Receptors Pathway antibodies	Eicosanoid Ligand-binding Receptors Pathway Diagram
G Alpha (s) Signalling Events Pathway antibodies	G Alpha (s) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
GPCRs, Class A Rhodopsin-like Pathway antibodies	GPCRs, Class A Rhodopsin-like Pathway Diagram
Inflammatory Mediator Regulation Of TRP Channels Pathway antibodies	Inflammatory Mediator Regulation Of TRP Channels Pathway Diagram
Inflammatory Mediator Regulation Of TRP Channels Pathway antibodies	Inflammatory Mediator Regulation Of TRP Channels Pathway Diagram
Neuroactive Ligand-receptor Interaction Pathway antibodies	Neuroactive Ligand-receptor Interaction Pathway Diagram
Neuroactive Ligand-receptor Interaction Pathway antibodies	Neuroactive Ligand-receptor Interaction Pathway Diagram

Diseases associated with PG-E2 elisa kit

Disease Name	Pubmed Publications
Inflammation Antibodies	>90 publications with PG-E2 and Inflammation
Disease Models, Animal Antibodies	>61 publications with PG-E2 and Disease Models, Animal
Necrosis Antibodies	>50 publications with PG-E2 and Necrosis
Cardiovascular Diseases Antibodies	>43 publications with PG-E2 and Cardiovascular Diseases
Heart Diseases Antibodies	>17 publications with PG-E2 and Heart Diseases
Kidney Diseases Antibodies	>15 publications with PG-E2 and Kidney Diseases
Neoplasms, Experimental Antibodies	>14 publications with PG-E2 and Neoplasms, Experimental
Breast Neoplasms Antibodies	>14 publications with PG-E2 and Breast Neoplasms
Pain Antibodies	>11 publications with PG-E2 and Pain
Lung Neoplasms Antibodies	>10 publications with PG-E2 and Lung Neoplasms

Organs/Tissues associated with PG-E2 elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>131 publications with PG-E2 and Blood
Vascular Antibodies	>63 publications with PG-E2 and Vascular
Kidney Antibodies	>44 publications with PG-E2 and Kidney
Brain Antibodies	>40 publications with PG-E2 and Brain
Lung Antibodies	>33 publications with PG-E2 and Lung
Intestine Antibodies	>31 publications with PG-E2 and Intestine
Eye Antibodies	>22 publications with PG-E2 and Eye
Connective Tissue Antibodies	>19 publications with PG-E2 and Connective Tissue
Liver Antibodies	>19 publications with PG-E2 and Liver
Skin Antibodies	>16 publications with PG-E2 and Skin