Melanin-Concentrating Hormone Receptor 1 (MCHR1) ELISA Kit for Monkey

Melanin-concentrating hormone receptor 1 is one of the melanin-concentrating hormone receptors.The protein encoded by this gene, a member of the G protein-coupled receptor family 1, is an integral plasma membrane protein which binds melanin-concentrating hormone. The encoded protein can inhibit cAMP accumulation and stimulate intracellular calcium flux, and is probably involved in the neuronal regulation of food consumption. Although structurally similar to somatostatin receptors, this protein does not seem to bind somatostatin.MCH1 is thought to have a number of functions including in the regulation of appetite, and in stress, anxiety and depression.Highest level in brain, particularly in the frontal cortex and hypothalamus, lower levels in the liver and heart.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MCHR1 in samples. An antibody specific for MCHR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMCHR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MCHR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MCHR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of MCHR1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS286013 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Melanin-concentrating hormone receptor 1 (MCHR1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MCHR1. The ELISA analytical biochemical technique of the MBS286013 kit is based on MCHR1 antibody-MCHR1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MCHR1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MCHR1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).