Zinc Finger Protein ZIC 1 (ZIC1) ELISA Kit for Human

Description: This assay employs a two-site sandwich ELISA to quantitate ZIC1 in samples. An antibody specific for ZIC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ZIC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZIC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZIC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: The putative open reading frame is 1,344 nucleotides encoding 447 amino acids and is preceded by 530 nucleotides of a 5-prime untranslated region. Northern blot hybridization studies failed to demonstrate zic expression in any tissues other than nervous tissue. Within the central nervous system, the highest level of expression was in the cerebellum, whereas the olfactory bulb, diencephalon, and brainstem showed only low levels of zic expression. The zic gene has significant homology to the zinc finger domain of the Caenorhabditis elegans tra1 gene, the Drosophila cubitus interruptus dominant gene, and the human GLI oncogene. In situ hybridization demonstrated restricted expression of zic in granule cells and their putative precursors. It is also expressed in the dorsal half of the neural tube at early embryonic stages.

Small volumes of ZIC1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS286583 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Zinc finger protein ZIC 1 (ZIC1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ZIC1. The ELISA analytical biochemical technique of the MBS286583 kit is based on ZIC1 antibody-ZIC1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ZIC1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ZIC1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).