Zinc Finger C3H1 Domain-Containing (ZFC3H1) ELISA Kit for Human

Description: This assay employs a two-site sandwich ELISA to quantitate ZFC3H1 in samples. An antibody specific for ZFC3H1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ZFC3H1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZFC3H1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZFC3H1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: ZFC3H1 May be involved in transcriptional regulation.Contains 1 C3H1-type zinc finger. Contains 6 HAT repeats.Contains 5 TPR repeats.
A zinc finger protein is a DNA-binding protein domain consisting of zinc fingers ranging from two in the Drosophila regulator ADR1, the more common three in mammalian Sp1 up to nine in TFIIIA. They occur in nature as the part of transcription factors conferring DNA sequence specificity as the DNA-binding domain. They have also found use in protein engineering due to their modularity and have prospects as components of tools for use in therapeutic gene modulation and zinc finger nucleases. Zinc finger protein consists of anti-parallel hairpin motif. It consists of 2 beta strands, one alpha helix and a hairpin structure.

Small volumes of ZFC3H1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS286595 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Zinc finger C3H1 domain-containing protein (ZFC3H1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ZFC3H1. The ELISA analytical biochemical technique of the MBS286595 kit is based on ZFC3H1 antibody-ZFC3H1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ZFC3H1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ZFC3H1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).