Nuclear-Interacting Partner of ALK (ZC3HC1) ELISA Kit for Human

Description: This assay employs a two-site sandwich ELISA to quantitate ZC3HC1 in samples. An antibody specific for ZC3HC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ZC3HC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZC3HC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZC3HC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: NIPA, Contains 1 C3HC-type zinc finger.Widely expressed. Highly expressed in heart, skeletal muscle and testis. Expressed in brain, placenta, lung, kidney, liver, pancreas, spleen, thymus, prostate, ovary small intestine and colon. Weakly or not expressed in leukocytes.Essential component of an SCF-type E3 ligase complex, SCF(NIPA), a complex that controls mitotic entry by mediating ubiquitination and subsequent degradation of cyclin B1 (CCNB1). Its cell-cycle-dependent phosphorylation regulates the assembly of the SCF(NIPA) complex, restricting CCNB1 ubiquitination activity to interphase. Its inactivation results in nuclear accumulation of CCNB1 in interphase and premature mitotic entry. May have an antiapoptotic role in NPM-ALK-mediated signaling events.

Small volumes of ZC3HC1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS286600 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Nuclear-interacting partner of ALK (ZC3HC1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ZC3HC1. The ELISA analytical biochemical technique of the MBS286600 kit is based on ZC3HC1 antibody-ZC3HC1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ZC3HC1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ZC3HC1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).