DNA Repair Protein XRCC4 (XRCC4) ELISA Kit for Human

Description: This assay employs a two-site sandwich ELISA to quantitate XRCC4 in samples. An antibody specific for XRCC4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any XRCC4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for XRCC4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of XRCC4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: X-Ray Repair Cross-Complementing 4 functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. The non-homologous end-joining pathway is required both for normal development and for suppression of tumors.
This gene functionally complements XR-1 Chinese hamster ovary cell mutant, which is impaired in DNA double-strand breaks produced by ionizing radiation and restriction enzymes. This gene contains 8 exons, and alternative transcription initiation and alternative splicing generates several transcript variants.

Small volumes of XRCC4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS286633 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the DNA repair protein XRCC4 (XRCC4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing XRCC4. The ELISA analytical biochemical technique of the MBS286633 kit is based on XRCC4 antibody-XRCC4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect XRCC4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, XRCC4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).