DNA Repair Protein XRCC1 (XRCC1) ELISA Kit for Human

Description: This assay employs a two-site sandwich ELISA to quantitate XRCC1 in samples. An antibody specific for XRCC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any XRCC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for XRCC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of XRCC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: XRCC1 is a DNA repair protein.involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.Inhibition of XRCC1 phosphorylation by mutation of the CK2 phosphorylation sites or by preventing CK2 activity using a highly specific inhibitor ablated the rapid repair of cellular DNA single-strand breaks by XRCC1. These data identified a direct role for CK2 in the repair of chromosome DNA strand breaks and in maintaining genetic integrity.

Small volumes of XRCC1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS286635 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the DNA repair protein XRCC1 (XRCC1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing XRCC1. The ELISA analytical biochemical technique of the MBS286635 kit is based on XRCC1 antibody-XRCC1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect XRCC1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, XRCC1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).