Serine/Threonine-Protein Kinase VRK2 (VRK2) ELISA Kit for Human

Name: Human VRK2 ELISA Kit; Serine/Threonine-Protein Kinase VRK2; MyBioSource
Brand: MyBioSource
SKU: MBS286704
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS286704

This assay employs a two-site sandwich ELISA to quantitate VRK2 in samples. An antibody specific for VRK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VRK2 present is bound by the immobilized antibody. After… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Serine/threonine-protein kinase VRK2 (VRK2), ELISA Kit

Full Product Name

Human Serine/threonine-protein kinase VRK2 (VRK2) ELISA Kit

Product Gene Name

VRK2 elisa kit

Product Synonym Names

OTTHUMP00000201223; vaccinia virus B1R-related kinase 2; vaccinia-related kinase-2

Species Reactivity

Human

Assay Type

Quantitive Sandwich

Samples

Serum, Plasma, Other Biological Fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human VRK2. No significant cross-reactivity or interference between Human VRK2 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

45,034 Da

OMIM

AB000450 mRNA

3D Structure

ModBase 3D Structure for Q86Y07

Detection Wavelength

450 nm

Intra-assay Precision (Precision within an assay)

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

CV (%)

= SD/meanX100

Intra-Assay

Inter-Assay

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of VRK2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for VRK2 purchase

MBS286704 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Serine/threonine-protein kinase VRK2 (VRK2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing VRK2. The ELISA analytical biochemical technique of the MBS286704 kit is based on VRK2 antibody-VRK2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect VRK2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, VRK2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for VRK2. It may not necessarily be applicable to this product.

NCBI GI #

194440707

NCBI GeneID

7444

NCBI Accession #

NP_001123952.1

UniProt Primary Accession #

Q86Y07

UniProt Secondary Accession #

Q49AK9; Q53EU9; Q53S39; Q53S77; Q53TU1; Q86Y08; Q86Y09; Q86Y10; B4DKL0; D6W5D4; D6W5D6

UniProt Related Accession #

Q86Y07

GenBank Nucleotide #

NM_001130480.2

NCBI Official Full Name

serine/threonine-protein kinase VRK2 isoform 1

NCBI Official Synonym Full Names

vaccinia related kinase 2

NCBI Official Symbol

VRK2

UniProt Protein Name

Serine/threonine-protein kinase VRK2

UniProt Synonym Protein Names

Vaccinia-related kinase 2

Protein Family

Serine/threonine-protein kinase

UniProt Gene Name

VRK2

NCBI Protein Information

serine/threonine-protein kinase VRK2

NCBI Summary for VRK2

This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. The encoded protein acts as an effector of signaling pathways that regulate apoptosis and tumor cell growth. Variants in this gene have been associated with schizophrenia. Alternative splicing results in multiple transcript variants that differ in their subcellular localization and biological activity. [provided by RefSeq, Jan 2014]

UniProt Comments for VRK2

Serine/threonine kinase that regulates several signal transduction pathways. Isoform 1 modulates the stress response to hypoxia and cytokines, such as interleukin-1 beta (IL1B) and this is dependent on its interaction with MAPK8IP1, which assembles mitogen-activated protein kinase (MAPK) complexes. Inhibition of signal transmission mediated by the assembly of MAPK8IP1-MAPK complexes reduces JNK phosphorylation and JUN-dependent transcription. Phosphorylates 'Thr-18' of p53/TP53, histone H3, and may also phosphorylate MAPK8IP1. Phosphorylates BANF1 and disrupts its ability to bind DNA and reduces its binding to LEM domain-containing proteins. Downregulates the transactivation of transcription induced by ERBB2, HRAS, BRAF, and MEK1. Blocks the phosphorylation of ERK in response to ERBB2 and HRAS. Can also phosphorylate the following substrates that are commonly used to establish in vitro kinase activity: casein, MBP and histone H2B, but it is not sure that this is physiologically relevant.

Research Articles on VRK2

1. The combined analysis confirmed evidence of genome-wide significant associations in the Han Chinese population for three loci, at 2p16.1 (rs1051061, in an exon of VRK2, P=1.14 x 10-12, odds ratio (OR)=1.17), 6p22.1 (rs115070292 in an intron of GABBR1, P=4.96 x 10-10, OR=0.77) and 10q24.32 (rs10883795 in an intron of AS3MT, P=7.94 x 10-10, OR=0.87; rs10883765 at an intron of ARL3, P=3.06 x 10-9, OR=0.87).

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with VRK2 elisa kit

Reference Product	PubMed Publications
MAPK8IP1 elisa kit	>3 publications with VRK2 and MAPK8IP1
BANF1 elisa kit	>3 publications with VRK2 and BANF1

Pathways associated with VRK2 elisa kit

Products by Pathway	Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle, Mitotic Pathway antibodies	Cell Cycle, Mitotic Pathway Diagram
Clearance Of Nuclear Envelope Membranes From Chromatin Pathway antibodies	Clearance Of Nuclear Envelope Membranes From Chromatin Pathway Diagram
Initiation Of Nuclear Envelope Reformation Pathway antibodies	Initiation Of Nuclear Envelope Reformation Pathway Diagram
M Phase Pathway antibodies	M Phase Pathway Diagram
Mitotic Anaphase Pathway antibodies	Mitotic Anaphase Pathway Diagram
Mitotic Metaphase And Anaphase Pathway antibodies	Mitotic Metaphase And Anaphase Pathway Diagram
Mitotic Prophase Pathway antibodies	Mitotic Prophase Pathway Diagram
Nuclear Envelope Breakdown Pathway antibodies	Nuclear Envelope Breakdown Pathway Diagram
Nuclear Envelope Reassembly Pathway antibodies	Nuclear Envelope Reassembly Pathway Diagram

Diseases associated with VRK2 elisa kit

Disease Name	Pubmed Publications
Neoplasms, Experimental Antibodies	>3 publications with VRK2 and Neoplasms, Experimental
Breast Neoplasms Antibodies	>2 publications with VRK2 and Breast Neoplasms
Cognition Disorders Antibodies	>2 publications with VRK2 and Cognition Disorders
Kidney Diseases Antibodies	>2 publications with VRK2 and Kidney Diseases
Nervous System Malformations Antibodies	>1 publications with VRK2 and Nervous System Malformations
Lung Diseases Antibodies	>1 publications with VRK2 and Lung Diseases
Neoplasm Metastasis Antibodies	>1 publications with VRK2 and Neoplasm Metastasis
Lung Neoplasms Antibodies	>1 publications with VRK2 and Lung Neoplasms
Liver Diseases Antibodies	>1 publications with VRK2 and Liver Diseases
Liver Neoplasms Antibodies	>1 publications with VRK2 and Liver Neoplasms

Organs/Tissues associated with VRK2 elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>10 publications with VRK2 and Brain
Testis Antibodies	>2 publications with VRK2 and Testis
Kidney Antibodies	>2 publications with VRK2 and Kidney