• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Glutamic Acid Decarboxylase (GAD) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS287203

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GAD in Human serum, plasma. An antibody specific for GAD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GAD present is bound… More details

$545 / 48 Tests +1 FREE 8GB USB

Product Documentation

PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Glutamic acid decarboxylase (GAD), ELISA Kit
Full Product Name
Human Glutamic acid decarboxylase (GAD) ELISA Kit
Product Gene Name
GAD elisa kit
Product Synonym Gene Name
GAD1
Product Synonym Names
FLJ45882; GAD; SCP; glutamate decarboxylase 1
Species Reactivity
Human
Assay Type
Quantitative Sandwich
Samples
Serum, plasma and other biological fluids
Detection Range
0.31 ng/mL - 20 ng/mL
Sensitivity
0.1 ng/mL (mean of 6 independent assays)
Specificity
This assay has high sensitivity and excellent specificity for detection of Human GAD. No significant cross-reactivity or interference between Human GAD and analogues was observed.
Preparation and Storage
Store at 2-8 degree C.
Inter-assay Precision
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Intra-assay Precision
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Molecular Weight
47,440 Da
OMIM
M81883 mRNA
3D Structure
ModBase 3D Structure for Q99259
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of GAD elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GAD purchase

MBS287203 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Glutamic acid decarboxylase (GAD) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GAD. The ELISA analytical biochemical technique of the MBS287203 kit is based on GAD antibody-GAD antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GAD antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GAD. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for GAD. It may not necessarily be applicable to this product.

NCBI GI #
58331246
NCBI GeneID
2571
NCBI Accession #
NP_000808.2
UniProt Primary Accession #
Q99259
UniProt Secondary Accession #
Q49AK1; Q53TQ7; Q9BU91; Q9UHH4
UniProt Related Accession #
Q99259
GenBank Nucleotide #
NM_000817.2
NCBI Official Full Name
glutamate decarboxylase 1 isoform GAD67
NCBI Official Synonym Full Names
glutamate decarboxylase 1
NCBI Official Symbol
GAD1
NCBI Official Synonym Symbols
GAD; SCP; CPSQ1
UniProt Protein Name
Glutamate decarboxylase 1
UniProt Synonym Protein Names
67 kDa glutamic acid decarboxylase; GAD-67; Glutamate decarboxylase 67 kDa isoform
Protein Family
D-gluconate/D-galactonate dehydratase
UniProt Gene Name
GAD1
UniProt Synonym Gene Names
GAD; GAD67; GAD-67
NCBI Protein Information
glutamate decarboxylase 1

NCBI Summary for GAD

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq, Jul 2008]
Show more

UniProt Comments for GAD

Catalyzes the production of GABA.

Research Articles on GAD

1. findings converge on variation in glutamic acid decarboxylase 1 gene predicting a susceptibility mechanism that affects white matter fractional anisotropy, GABAergic inhibitory neurotransmission in the dorsolateral prefrontal cortex, and working memory performance.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GAD elisa kit

 Reference Product  PubMed Publications
 GAD2 elisa kit  >74 publications with GAD and GAD2
 GLUL elisa kit  >3 publications with GAD and GLUL
 GLS elisa kit  >2 publications with GAD and GLS
 ABAT elisa kit  >2 publications with GAD and ABAT
 SLC32A1 elisa kit  >2 publications with GAD and SLC32A1
 SNAP25 elisa kit  >1 publications with GAD and SNAP25

Pathways associated with GAD elisa kit

 Products by Pathway  Pathway Diagram
 Alanine And Aspartate Metabolism Pathway antibodies  Alanine And Aspartate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Biogenic Amine Synthesis Pathway antibodies  Biogenic Amine Synthesis Pathway Diagram
 Butanoate Metabolism Pathway antibodies  Butanoate Metabolism Pathway Diagram
 Butanoate Metabolism Pathway antibodies  Butanoate Metabolism Pathway Diagram
 GABA (gamma-Aminobutyrate) Shunt Pathway antibodies  GABA (gamma-Aminobutyrate) Shunt Pathway Diagram
 GABA (gamma-Aminobutyrate) Shunt Pathway antibodies  GABA (gamma-Aminobutyrate) Shunt Pathway Diagram
 GABA Synthesis Pathway antibodies  GABA Synthesis Pathway Diagram
 GABA Synthesis, Release, Reuptake And Degradation Pathway antibodies  GABA Synthesis, Release, Reuptake And Degradation Pathway Diagram

Diseases associated with GAD elisa kit

 Disease Name  Pubmed Publications
 Mental Disorders Antibodies  >83 publications with GAD and Mental Disorders
 Schizophrenia Antibodies  >59 publications with GAD and Schizophrenia
 Nervous System Diseases Antibodies  >39 publications with GAD and Nervous System Diseases
 Disease Models, Animal Antibodies  >30 publications with GAD and Disease Models, Animal
 Brain Diseases Antibodies  >29 publications with GAD and Brain Diseases
 Bipolar Disorder Antibodies  >19 publications with GAD and Bipolar Disorder
 Seizures Antibodies  >10 publications with GAD and Seizures
 Anxiety Disorders Antibodies  >9 publications with GAD and Anxiety Disorders
 Depressive Disorder Antibodies  >8 publications with GAD and Depressive Disorder
 Prenatal Exposure Delayed Effects Antibodies  >7 publications with GAD and Prenatal Exposure Delayed Effects

Organs/Tissues associated with GAD elisa kit

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >156 publications with GAD and Brain
 Liver Antibodies  >4 publications with GAD and Liver
 Eye Antibodies  >3 publications with GAD and Eye
 Bone Antibodies  >2 publications with GAD and Bone
 Embryonic Tissue Antibodies  >2 publications with GAD and Embryonic Tissue
 Muscle Antibodies  >2 publications with GAD and Muscle
 Kidney Antibodies  >2 publications with GAD and Kidney
 Thymus Antibodies  >1 publications with GAD and Thymus
 Uncharacterized Tissue Antibodies  >1 publications with GAD and Uncharacterized Tissue
 Intestine Antibodies  >1 publications with GAD and Intestine
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000