Metallophosphoesterase 1 (MPPE1) ELISA Kit for Human

Description: This assay employs a two-site sandwich ELISA to quantitate MPPE1 in samples. An antibody specific for MPPE1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MPPE1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MPPE1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MPPE1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: MPPE1 gene contains 14 exons and spans about 27 kb. They detected 4 alternatively spliced forms: the most abundant form contains exons 1, 2, 5-12, and 14, and another form consists of exons 1, 2, and 5-14; the 2 rare forms correspond to these 2, but lack the sequences for exon 10.
The most abundant form encodes a deduced 396-amino acid protein with a signal sequence, a metallophosphoesterase domain, and a putative transmembrane domain. One of the alternative forms encodes a truncated 340-amino acid protein. Northern blot analysis detected a single 2.2-kb MPPE1 mRNA in brain, but not in any other tissues tested. The 3 alternative forms were detected in brain by RT-PCR only.

Small volumes of MPPE1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS287538 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Metallophosphoesterase 1 (MPPE1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MPPE1. The ELISA analytical biochemical technique of the MBS287538 kit is based on MPPE1 antibody-MPPE1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MPPE1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MPPE1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).