Hepatitis B Virus X Interacting (HBXIP) ELISA Kit for Human

Description: This assay employs a two-site sandwich ELISA to quantitate HBXIP in samples. An antibody specific for HBXIP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HBXIP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HBXIP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HBXIP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: HBXIP encodes a protein that specifically complexes with the C-terminus of hepatitis B virus X protein (HBx). The function of this protein is to negatively regulate HBx activity and thus to alter the replication life cycle of the virus.
The deduced 91-amino acid protein has a calculated molecular mass of 9.6 kD. It contains a putative leucine zipper motif and may form an amphipathic helix. HBXIP also has 2 putative threonine phosphorylation sites. Northern blot analysis detected a 0.7-kb transcript in all tissues tested, with highest expression in skeletal and cardiac muscle.HBXIP expression in differentiated hepatocellular carcinoma cells reduced wildtype HBV replication to levels observed following infection with an HBX-minus virus.

Small volumes of HBXIP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS287609 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Hepatitis B virus X interacting protein (HBXIP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HBXIP. The ELISA analytical biochemical technique of the MBS287609 kit is based on HBXIP antibody-HBXIP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HBXIP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HBXIP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).