Frizzled-9 (FZD9) ELISA Kit for Human

Description: This assay employs a two-site sandwich ELISA to quantitate FZD9 in samples. An antibody specific for FZD9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FZD9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FZD9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FZD9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: The FZD9 gene is located within the Williams syndrome common deletion region of chromosome 7, and heterozygous deletion of the FZD9 gene may contribute to the Williams syndrome phenotype. FZD9 is expressed predominantly in brain, testis, eye, skeletal muscle, and kidney. In mouse, the wg-homologous Wnt1 gene is involved in early development of a large domain of the central nervous system encompassing much of the midbrain and rostral metencephalon.The potential function of FZD9 in transmitting a Wnt protein signal in the human brain and other tissues suggested to Wang et al. (1997) that heterozygous deletion of the FZD9 gene could contribute to the Williams syndrome phenotype.

Small volumes of FZD9 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS287651 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Frizzled-9 (FZD9) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FZD9. The ELISA analytical biochemical technique of the MBS287651 kit is based on FZD9 antibody-FZD9 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FZD9 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FZD9. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).