Filaggrin-2 (FLG2) ELISA Kit for Human

Description: This assay employs a two-site sandwich ELISA to quantitate FLG2 in samples. An antibody specific for FLG2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FLG2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FLG2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FLG2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: The presence of ifapsoriasin (IFPS, fillagrin-2), a novel skin- and mucosaspecific S100 fusion type protein, at the surface of skin and mucosa, particularly where moisture-requiring soil bacteria may grow, reveals its potential as innate defense factor in the protection of body surfaces from infection with soil bacteria.
IFPS may be used in the therapy of infectious diseases of epithelial organs and for infection prophylaxis at interfaces, especially of the skin, the nasopharynx, the eyes as well as the respiratory, gastrointestinal and urogenital tracts. The filaggrin 2 is a new member of the EDC, a cluster of approximately 50 genes involved in cornification. filaggrin 2, a poorly characterized member of the epidermal differentiation complex, as well as three new lipase genes clustered with paralogous genes on chromosome 10q23.31.

Small volumes of FLG2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS287667 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Filaggrin-2 (FLG2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FLG2. The ELISA analytical biochemical technique of the MBS287667 kit is based on FLG2 antibody-FLG2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FLG2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FLG2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).