DNA-Directed RNA Polymerase III Subunit RPC6 (POLR3F) ELISA Kit for Mouse

This assay employs a two-site sandwich ELISA to quantitate POLR3F in samples. An antibody specific for POLR3F has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any POLR3F present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POLR3F is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POLR3F bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of POLR3F elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS287957 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the DNA-directed RNA polymerase III subunit RPC6 (POLR3F) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing POLR3F. The ELISA analytical biochemical technique of the MBS287957 kit is based on POLR3F antibody-POLR3F antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect POLR3F antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, POLR3F. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).