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Cgmp-Specific 3',5'-Cyclic Phosphodiesterase (PDE5A) ELISA Kit for Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS288092

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.Principle of the Assay: The ELISA is based on the… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
cGMP-specific 3',5'-cyclic phosphodiesterase (Pde5a), ELISA Kit
Full Product Name
Mouse cGMP-specific 3',5'-cyclic phosphodiesterase ELISA Kit
Product Gene Name
Pde5a elisa kit
Species Reactivity
Mouse
Assay Type
Quantitative Competitive
Samples
Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
Detection Range
0.625-40 ng/mL
Sensitivity
< 0.334 ng/mL
Specificity
Recombinant and natural Mouse cGMP-specific 3',5'-cyclic phosphodiesterase
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Inter-assay Precision
Inter-Assay CV: <=8.5%
Intra-assay Precision
Intra-Assay CV: <=6.8%
Molecular Weight
98,407 Da
3D Structure
ModBase 3D Structure for Q8CG03

Typical Testing Data/Standard Curve (for reference only) of Pde5a elisa kit

Mouse PDE5A ELISA Kit – Cat# MBS288092

Other Notes

Small volumes of Pde5a elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Pde5a purchase

MBS288092 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the cGMP-specific 3',5'-cyclic phosphodiesterase (Pde5a) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Pde5a. The ELISA analytical biochemical technique of the MBS288092 kit is based on Pde5a antibody-Pde5a antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Pde5a antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Pde5a. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for Pde5a. It may not necessarily be applicable to this product.

NCBI GI #
260166631
NCBI GeneID
242202
NCBI Accession #
NP_700471.2
UniProt Primary Accession #
Q8CG03
UniProt Secondary Accession #
Q0VBW0
UniProt Related Accession #
Q8CG03
GenBank Nucleotide #
NM_153422.2
NCBI Official Full Name
cGMP-specific 3',5'-cyclic phosphodiesterase
NCBI Official Synonym Full Names
phosphodiesterase 5A, cGMP-specific
NCBI Official Symbol
Pde5a
NCBI Official Synonym Symbols
Cn5n; Pde5; Cgbpde; PDE5a2; Pde5a1
UniProt Protein Name
cGMP-specific 3',5'-cyclic phosphodiesterase
UniProt Synonym Protein Names
cGMP-binding cGMP-specific phosphodiesterase; CGB-PDE
Protein Family
cGMP-specific 3',5'-cyclic phosphodiesterase
UniProt Gene Name
Pde5a
UniProt Synonym Gene Names
Pde5; CGB-PDE
UniProt Entry Name
PDE5A_MOUSE
NCBI Protein Information
cGMP-specific 3',5'-cyclic phosphodiesterase

UniProt Comments for Pde5a

PDE5A: a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Phosphorylation is regulated by binding of cGMP to the two allosteric sites. Sildenafil (Viagra) is a highly selective and potent inhibitor of PDE5A. Alternative splicing results in four transcript variants encoding distinct isoforms.

Protein type: Nucleotide Metabolism - purine; EC 3.1.4.35; Phosphodiesterase

Cellular Component: cytosol

Molecular Function: 3',5'-cyclic-GMP phosphodiesterase activity; 3',5'-cyclic-nucleotide phosphodiesterase activity; catalytic activity; cGMP binding; cyclic-nucleotide phosphodiesterase activity; hydrolase activity; metal ion binding; nucleotide binding; phosphoric diester hydrolase activity

Biological Process: cGMP catabolic process; cGMP metabolic process; metabolic process; negative regulation of T cell proliferation; positive regulation of apoptosis; positive regulation of chronic inflammatory response; positive regulation of G-protein coupled receptor protein signaling pathway; positive regulation of MAP kinase activity; positive regulation of vasoconstriction; regulation of cGMP metabolic process; regulation of the force of heart contraction; short-term memory; signal transduction
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Research Articles on Pde5a

1. Inhibition of type 5 phosphodiesterase counteracts beta2-adrenergic signalling in beating cardiomyocytes.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with Pde5a elisa kit

 Reference Product  PubMed Publications
 Prkg1 elisa kit  >5 publications with Pde5a and Prkg1

Pathways associated with Pde5a elisa kit

 Products by Pathway  Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Nitric Oxide Stimulates Guanylate Cyclase Pathway antibodies  Nitric Oxide Stimulates Guanylate Cyclase Pathway Diagram
 Platelet Homeostasis Pathway antibodies  Platelet Homeostasis Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 CGMP Effects Pathway antibodies  CGMP Effects Pathway Diagram
 CGMP-PKG Signaling Pathway antibodies  CGMP-PKG Signaling Pathway Diagram
 CGMP-PKG Signaling Pathway antibodies  CGMP-PKG Signaling Pathway Diagram
 Cyclic GMP Biosynthesis Pathway antibodies  Cyclic GMP Biosynthesis Pathway Diagram

Diseases associated with Pde5a elisa kit

 Disease Name  Pubmed Publications
 Erectile Dysfunction Antibodies  >279 publications with Pde5a and Erectile Dysfunction
 Cardiovascular Diseases Antibodies  >153 publications with Pde5a and Cardiovascular Diseases
 Hypertension Antibodies  >124 publications with Pde5a and Hypertension
 Heart Diseases Antibodies  >97 publications with Pde5a and Heart Diseases
 Nervous System Diseases Antibodies  >45 publications with Pde5a and Nervous System Diseases
 Disease Models, Animal Antibodies  >40 publications with Pde5a and Disease Models, Animal
 Hypertrophy Antibodies  >36 publications with Pde5a and Hypertrophy
 Fibrosis Antibodies  >20 publications with Pde5a and Fibrosis
 Brain Diseases Antibodies  >19 publications with Pde5a and Brain Diseases
 Inflammation Antibodies  >16 publications with Pde5a and Inflammation

Organs/Tissues associated with Pde5a elisa kit

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >227 publications with Pde5a and Muscle
 Other Antibodies  >108 publications with Pde5a and Other
 Brain Antibodies  >55 publications with Pde5a and Brain
 Lung Antibodies  >54 publications with Pde5a and Lung
 Testis Antibodies  >16 publications with Pde5a and Testis
 Eye Antibodies  >11 publications with Pde5a and Eye
 Bladder Antibodies  >9 publications with Pde5a and Bladder
 Intestine Antibodies  >8 publications with Pde5a and Intestine
 Skin Antibodies  >7 publications with Pde5a and Skin
 Pancreas Antibodies  >6 publications with Pde5a and Pancreas
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