Probable 4-Hydroxy-2-Oxoglutarate Aldolase, Mitochondrial (HOGA1) ELISA Kit for Human

Name: Human HOGA1 ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS288132
Price: 405 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS288132

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Probable 4-hydroxy-2-oxoglutarate aldolase, mitochondrial (HOGA1), ELISA Kit

Full Product Name

Human Probable 4-hydroxy-2-oxoglutarate aldolase, mitochondrial ELISA Kit

Product Gene Name

HOGA1 elisa kit

Species Reactivity

Human

Samples

Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids

Detection Range

0.312-20 ng/mL

Sensitivity

< 0.1 ng/mL

Specificity

Recombinant and natural Human 4-hydroxy-2-oxoglutarate aldolase, mitochondrial

Preparation and Storage

For long term storage, please store the entire kit at -20 degree C.

Inter-assay Precision

<=10.1%

Intra-assay Precision

<=5.3%

Molecular Weight

17,954 Da

OMIM

613597

3D Structure

ModBase 3D Structure for Q86XE5

Other Notes

Small volumes of HOGA1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HOGA1 purchase

MBS288132 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Probable 4-hydroxy-2-oxoglutarate aldolase, mitochondrial (HOGA1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HOGA1. The ELISA analytical biochemical technique of the MBS288132 kit is based on HOGA1 antibody-HOGA1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HOGA1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HOGA1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for HOGA1. It may not necessarily be applicable to this product.

NCBI GI #

197927274

NCBI GeneID

112817

NCBI Accession #

NP_001128142.1

UniProt Primary Accession #

Q86XE5

UniProt Secondary Accession #

Q5T680; Q5T684; Q711P0; Q8N9F2; Q96EV5; A8K075

UniProt Related Accession #

Q86XE5

GenBank Nucleotide #

NM_001134670.1

NCBI Official Full Name

4-hydroxy-2-oxoglutarate aldolase, mitochondrial isoform 2

NCBI Official Synonym Full Names

4-hydroxy-2-oxoglutarate aldolase 1

NCBI Official Symbol

HOGA1

NCBI Official Synonym Symbols

HP3; NPL2; DHDPS2; DHDPSL; C10orf65

UniProt Protein Name

4-hydroxy-2-oxoglutarate aldolase, mitochondrial

UniProt Synonym Protein Names

Dihydrodipicolinate synthase-like; DHDPS-like protein; Probable 2-keto-4-hydroxyglutarate aldolase; Probable KHG-aldolase; Protein 569272

Protein Family

4-hydroxy-2-oxoglutarate aldolase

UniProt Gene Name

HOGA1

UniProt Synonym Gene Names

C10orf65; DHDPSL; DHDPS-like protein; Probable KHG-aldolase

UniProt Entry Name

HOGA1_HUMAN

NCBI Protein Information

4-hydroxy-2-oxoglutarate aldolase, mitochondrial

NCBI Summary for HOGA1

The authors of PMID:20797690 cloned this gene while searching for genes in a region of chromosome 10 linked to primary hyperoxalurea type III. They noted that even though the encoded protein has been described as a mitochondrial dihydrodipicolinate synthase-like enzyme, it shares little homology with E. coli dihydrodipicolinate synthase (Dhdps), particularly in the putative substrate-binding region. Moreover, neither lysine biosynthesis nor sialic acid metabolism, for which Dhdps is responsible, occurs in vertebrate mitochondria. They propose that this gene encodes mitochondrial 4-hydroxyl-2-oxoglutarate aldolase (EC 4.1.3.16), which catalyzes the final step in the metabolic pathway of hydroxyproline, releasing glyoxylate and pyruvate. This gene is predominantly expressed in the liver and kidney, and mutations in this gene are found in patients with primary hyperoxalurea type III. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Nov 2010]

UniProt Comments for HOGA1

DHDPSL: Catalyzes the final step in the metabolic pathway of hydroxyproline (Probable). Defects in HOGA1 are the cause of hyperoxaluria primary type 3 (HP3); also known as primary hyperoxaluria type III (PH3). HP3 is a disorder where the main clinical manifestation is calcium oxalate nephrolithiasis though chronic as well as terminal renal insufficiency has been described. It is characterized by an elevated urinary excretion of oxalate and L- glycerate. Belongs to the DHDPS family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Lyase; EC 4.1.3.16

Chromosomal Location of Human Ortholog: 10q24.2

Cellular Component: mitochondrion

Molecular Function: 4-hydroxy-2-oxoglutarate aldolase activity; protein homodimerization activity

Biological Process: 4-hydroxyproline catabolic process; glyoxylate catabolic process; glyoxylate metabolic process; oxalate metabolic process; pyruvate biosynthetic process

Disease: Hyperoxaluria, Primary, Type Iii

Research Articles on HOGA1

1. Our results strongly suggest HOGA1 as a major cause of PH, indicate a greater genetic heterogeneity of hyperoxaluria, and point to a favorable outcome of type III in the context of PH despite incomplete or absent biochemical remission

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with HOGA1 elisa kit

Reference Product	PubMed Publications
AGXT elisa kit	>4 publications with HOGA1 and AGXT
GRHPR elisa kit	>3 publications with HOGA1 and GRHPR

Pathways associated with HOGA1 elisa kit

Products by Pathway	Pathway Diagram
4-hydroxyproline Degradation Pathway antibodies	4-hydroxyproline Degradation Pathway Diagram
Arginine And Proline Metabolism Pathway antibodies	Arginine And Proline Metabolism Pathway Diagram
Arginine And Proline Metabolism Pathway antibodies	Arginine And Proline Metabolism Pathway Diagram
Glyoxylate And Dicarboxylate Metabolism Pathway antibodies	Glyoxylate And Dicarboxylate Metabolism Pathway Diagram
Glyoxylate And Dicarboxylate Metabolism Pathway antibodies	Glyoxylate And Dicarboxylate Metabolism Pathway Diagram
Metabolic Pathways antibodies	Metabolic Pathways Diagram
Trans-4-hydroxy-L-proline Degradation I Pathway antibodies	Trans-4-hydroxy-L-proline Degradation I Pathway Diagram

Diseases associated with HOGA1 elisa kit

Disease Name	Pubmed Publications
Kidney Diseases Antibodies	>5 publications with HOGA1 and Kidney Diseases
HYPEROXALURIA, PRIMARY, TYPE III Antibodies	>4 publications with HOGA1 and HYPEROXALURIA, PRIMARY, TYPE III
Hypertension Antibodies	>2 publications with HOGA1 and Hypertension

Organs/Tissues associated with HOGA1 elisa kit

Organ/Tissue Name	Pubmed Publications
Kidney Antibodies	>4 publications with HOGA1 and Kidney