Apoptosis-Inducing Factor 1, Mitochondrial (AIFM1) ELISA Kit for Mouse

PDCD8: Probable oxidoreductase that has a dual role in controlling cellular life and death; during apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e., caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase- independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner. Interacts with XIAP/BIRC4. Interacts (via N-terminus) with EIF3G (via C-terminus). Belongs to the FAD-dependent oxidoreductase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Apoptosis; EC 1.-.-.-; Oxidoreductase

Cellular Component: cytoplasm; cytosol; membrane; mitochondrial inner membrane; mitochondrial intermembrane space; mitochondrial outer membrane; mitochondrion; nucleus

Molecular Function: (R)-2-hydroxyglutarate dehydrogenase activity; (R)-2-hydroxyisocaproate dehydrogenase activity; 2-hydroxytetrahydrofuran dehydrogenase activity; 3-ketoglucose-reductase activity; 5-exo-hydroxycamphor dehydrogenase activity; acetoin dehydrogenase activity; aldo-keto reductase activity; arabinose reductase activity; C-3 sterol dehydrogenase (C-4 sterol decarboxylase) activity; D-arabinitol dehydrogenase (NADP+) activity; DNA binding; electron-transferring-flavoprotein dehydrogenase activity; epoxide dehydrogenase activity; FAD binding; gluconate dehydrogenase activity; isocitrate dehydrogenase activity; mevaldate reductase activity; NAD(P)H oxidase activity; oxidoreductase activity; oxidoreductase activity, acting on NADH or NADPH; phenylcoumaran benzylic ether reductase activity; protein binding; protein dimerization activity; steroid dehydrogenase activity; steroid dehydrogenase activity, acting on the CH-CH group of donors; steroid dehydrogenase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; xylose reductase activity

Biological Process: apoptosis; apoptotic mitochondrial changes; caspase activation; cell redox homeostasis; mitochondrial respiratory chain complex I assembly; neuron apoptosis; neuron differentiation; positive regulation of apoptosis; positive regulation of neuron apoptosis; response to oxidative stress