E3 Ubiquitin-Protein Ligase HACE1 (HACE1) ELISA Kit for Mouse

HACE1: E3 ubiquitin-protein ligase involved in Golgi membrane fusion and regulation of small GTPases. Acts as a regulator of Golgi membrane dynamics during the cell cycle: recruited to Golgi membrane by Rab proteins and regulates postmitotic Golgi membrane fusion. Acts by mediating ubiquitination during mitotic Golgi disassembly, ubiquitination serving as a signal for Golgi reassembly later, after cell division. Specifically interacts with GTP-bound RAC1, mediating ubiquitination and subsequent degradation of active RAC1, thereby playing a role in host defense against pathogens. May also act as a transcription regulator via its interaction with RARB. Defects in HACE1 are a cause of Wilms tumor (WT). WT is a pediatric malignancy of kidney and one of the most common solid cancers in childhood. HACE1 is epigenetically down-regulated in sporadic Wilms tumor. Moreover, a t(5;6)(q21;q21) translocation that truncates HACE1 has been found in a child with bilateral, young-onset Wilms tumor. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligase; Ubiquitin conjugating system; Ubiquitin ligase; EC 6.3.2.-; EC 6.3.2.19

Cellular Component: cytoplasm; endoplasmic reticulum; Golgi apparatus; Golgi membrane; membrane

Molecular Function: coenzyme F420-0 gamma-glutamyl ligase activity; coenzyme F420-2 alpha-glutamyl ligase activity; ligase activity; Rab GTPase binding; Rac GTPase binding; ribosomal S6-glutamic acid ligase activity; ubiquitin-protein ligase activity; UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity

Biological Process: cell cycle; Golgi organization and biogenesis; protein ubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process; regulation of cell migration; regulation of transcription, DNA-dependent; transcription, DNA-dependent