Galanin Peptides (GAL) ELISA Kit for Mouse

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of mouse Galanin peptides concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Galanin peptides, During the reaction, Galanin peptides in the sample or standard competes with a fixed amount of biotin-labeled Galanin peptides for sites on a pre-coated Monoclonal antibody specific to Galanin peptides. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Galanin peptides in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Small volumes of Gal elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS288839 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Galanin peptides, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Gal. The ELISA analytical biochemical technique of the MBS288839 kit is based on Gal antibody-Gal antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Gal antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Gal. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).