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DNA Topoisomerase 1 (TOP1) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS289119

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.Principle of the Assay: The microtiter plate… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DNA topoisomerase 1 (TOP1), ELISA Kit
Full Product Name
Human DNA topoisomerase 1 ELISA Kit
Product Gene Name
TOP1 elisa kit
Product Synonym Names
DNA topoisomerase 1; DNA topoisomerase I; TOP1; 5.99.1.2
Species Reactivity
Human
Samples
Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
Detection Range
0.78-50 ng/mL
Sensitivity
< 0.37 ng/mL
Specificity
Recombinant and natural Human DNA topoisomerase 1
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Inter-assay Precision
<=7.4%
Intra-assay Precision
<=4.5%
Molecular Weight
90,726 Da
OMIM
126420
3D Structure
ModBase 3D Structure for P11387

Typical Testing Data/Standard Curve (for reference only) of TOP1 elisa kit

Human TOP1 ELISA Kit – Cat# MBS289119

Other Notes

Small volumes of TOP1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TOP1 purchase

MBS289119 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the DNA topoisomerase 1 (TOP1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TOP1. The ELISA analytical biochemical technique of the MBS289119 kit is based on TOP1 antibody-TOP1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TOP1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TOP1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for TOP1. It may not necessarily be applicable to this product.

NCBI GI #
11225260
NCBI GeneID
7150
NCBI Accession #
NP_003277.1
UniProt Primary Accession #
P11387
UniProt Secondary Accession #
O43256; Q12855; Q12856; Q15610; Q5TFY3; Q9UJN0; A8KA78; E1P5W3
UniProt Related Accession #
P11387
GenBank Nucleotide #
NM_003286.2
NCBI Official Full Name
DNA topoisomerase 1
NCBI Official Synonym Full Names
topoisomerase (DNA) I
NCBI Official Symbol
TOP1
NCBI Official Synonym Symbols
TOPI
UniProt Protein Name
DNA topoisomerase 1
UniProt Synonym Protein Names
DNA topoisomerase I
Protein Family
DNA topoisomerase
UniProt Gene Name
TOP1
UniProt Entry Name
TOP1_HUMAN
NCBI Protein Information
DNA topoisomerase 1

NCBI Summary for TOP1

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq, Jul 2008]
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UniProt Comments for TOP1

TOP1: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98. Belongs to the type IB topoisomerase family.

Protein type: Nucleolus; Oncoprotein; EC 5.99.1.2; Isomerase

Chromosomal Location of Human Ortholog: 20q12-q13.1

Cellular Component: nuclear chromosome; nucleolus; nucleoplasm; nucleus; perikaryon

Molecular Function: chromatin binding; DNA binding; DNA topoisomerase (ATP-hydrolyzing) activity; DNA topoisomerase type I activity; protein binding

Biological Process: cellular protein metabolic process; chromatin remodeling; chromosome segregation; circadian regulation of gene expression; circadian rhythm; DNA replication; DNA topological change; embryonic cleavage; phosphorylation; post-translational protein modification; programmed cell death; protein sumoylation; response to drug; viral reproduction
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Research Articles on TOP1

1. Topoisomerase-1 and -2A gene copy numbers are elevated in mismatch repair-proficient colorectal cancers.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with TOP1 elisa kit

 Reference Product  PubMed Publications
 TDP1 elisa kit  >61 publications with TOP1 and TDP1
 TOP2A elisa kit  >9 publications with TOP1 and TOP2A
 CASP3 elisa kit  >5 publications with TOP1 and CASP3
 TP53 elisa kit  >5 publications with TOP1 and TP53
 TOP2B elisa kit  >4 publications with TOP1 and TOP2B
 UBC elisa kit  >2 publications with TOP1 and UBC
 BTBD2 elisa kit  >2 publications with TOP1 and BTBD2
 BTBD1 elisa kit  >2 publications with TOP1 and BTBD1
 SRSF1 elisa kit  >1 publications with TOP1 and SRSF1

Pathways associated with TOP1 elisa kit

 Products by Pathway  Pathway Diagram
 Caspase Cascade In Apoptosis Pathway antibodies  Caspase Cascade In Apoptosis Pathway Diagram
 Integrated Pancreatic Cancer Pathway antibodies  Integrated Pancreatic Cancer Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Post-translational Protein Modification Pathway antibodies  Post-translational Protein Modification Pathway Diagram
 SUMO E3 Ligases SUMOylate Target Proteins Pathway antibodies  SUMO E3 Ligases SUMOylate Target Proteins Pathway Diagram
 SUMOylation Pathway antibodies  SUMOylation Pathway Diagram
 SUMOylation Of DNA Replication Proteins Pathway antibodies  SUMOylation Of DNA Replication Proteins Pathway Diagram

Diseases associated with TOP1 elisa kit

 Disease Name  Pubmed Publications
 Leukemia Antibodies  >43 publications with TOP1 and Leukemia
 Poisoning Antibodies  >30 publications with TOP1 and Poisoning
 Breast Neoplasms Antibodies  >27 publications with TOP1 and Breast Neoplasms
 Adenocarcinoma Antibodies  >21 publications with TOP1 and Adenocarcinoma
 Nervous System Diseases Antibodies  >19 publications with TOP1 and Nervous System Diseases
 Neoplasms, Experimental Antibodies  >18 publications with TOP1 and Neoplasms, Experimental
 Brain Diseases Antibodies  >14 publications with TOP1 and Brain Diseases
 Kidney Diseases Antibodies  >8 publications with TOP1 and Kidney Diseases
 Prostatic Neoplasms Antibodies  >8 publications with TOP1 and Prostatic Neoplasms
 Neoplasm Metastasis Antibodies  >8 publications with TOP1 and Neoplasm Metastasis

Organs/Tissues associated with TOP1 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >41 publications with TOP1 and Blood
 Lung Antibodies  >40 publications with TOP1 and Lung
 Prostate Antibodies  >19 publications with TOP1 and Prostate
 Brain Antibodies  >15 publications with TOP1 and Brain
 Kidney Antibodies  >9 publications with TOP1 and Kidney
 Bone Antibodies  >9 publications with TOP1 and Bone
 Liver Antibodies  >9 publications with TOP1 and Liver
 Skin Antibodies  >8 publications with TOP1 and Skin
 Bone Marrow Antibodies  >8 publications with TOP1 and Bone Marrow
 Thymus Antibodies  >8 publications with TOP1 and Thymus
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