Eukaryotic Translation Initiation Factor 2a (EIF2A) ELISA Kit for Mouse

Name: Mouse EIF2A ELISA Kit; Eukaryotic Translation Initiation Factor 2a; MyBioSource
Brand: MyBioSource
SKU: MBS029011
Price: 675 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS029011

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated EIF2A concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse EIF2A antibody to coat Microelisa Stripplate… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Eukaryotic Translation Initiation Factor 2A (EIF2A), ELISA Kit

Full Product Name

Mouse Eukaryotic Translation Initiation Factor 2A ELISA Kit

Product Gene Name

EIF2A elisa kit

Species Reactivity

Mouse

Assay Type

Sandwich

Samples

Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids

Detection Range

62.5 pg/ml - 2000 pg/ml.

Sensitivity

10 pg/ml.

Specificity

No significant cross-reactivity or interference between Mouse EIF2A and analogues was observed.

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Intra-assay Precision

Intra-assay CV (%) is less than 15%.

Molecular Weight

64,990 Da

Chromosome Location

Chromosome: 3; NC_000003.11 (150264574..150303803). Location: 3q25.1

OMIM

609234

3D Structure

ModBase 3D Structure for Q9BY44

Intended Uses

This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated EIF2A concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse EIF2A antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add EIF2A and EIF2A antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of EIF2A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Other Notes

Small volumes of EIF2A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EIF2A purchase

MBS029011 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Eukaryotic Translation Initiation Factor 2A (EIF2A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing EIF2A. The ELISA analytical biochemical technique of the MBS029011 kit is based on EIF2A antibody-EIF2A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EIF2A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EIF2A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for EIF2A. It may not necessarily be applicable to this product.

NCBI GI #

54873624

NCBI GeneID

83939

NCBI Accession #

NP_114414.2

UniProt Primary Accession #

Q9BY44

UniProt Secondary Accession #

Q5QTR2; Q7Z4E9; Q8NFM1; Q96EW9; Q96K81; A8MPS6; D3DNI9

UniProt Related Accession #

Q9BY44

GenBank Nucleotide #

NM_032025.3

NCBI Official Full Name

eukaryotic translation initiation factor 2A

NCBI Official Synonym Full Names

eukaryotic translation initiation factor 2A, 65kDa

NCBI Official Symbol

EIF2A

NCBI Official Synonym Symbols

CDA02; EIF-2A; MST089; MSTP004; MSTP089

UniProt Protein Name

Eukaryotic translation initiation factor 2A

UniProt Synonym Protein Names

65 kDa eukaryotic translation initiation factor 2A

Protein Family

Eukaryotic translation initiation factor

UniProt Gene Name

EIF2A

UniProt Synonym Gene Names

eIF-2A

UniProt Entry Name

EIF2A_HUMAN

NCBI Protein Information

eukaryotic translation initiation factor 2A; 65 kDa eukaryotic translation initiation factor 2A

NCBI Summary for EIF2A

EIF2A is a 65-kD protein that catalyzes the formation of puromycin-sensitive 80S preinitiation complexes (Zoll et al., 2002 [PubMed 12133843]).[supplied by OMIM, Mar 2008]

UniProt Comments for EIF2A

EIF2A: Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF- 2 complex, it binds methionyl-tRNAi to 40 S subunits in a codon- dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40 S subunits in a GTP-dependent manner. May act by impiging the expression of specific proteins. Belongs to the WD repeat EIF2A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Translation

Chromosomal Location of Human Ortholog: 3q25.1

Cellular Component: extracellular space; cytoplasm; eukaryotic translation initiation factor 2 complex

Molecular Function: protein binding; translation initiation factor activity; ribosome binding; tRNA binding

Biological Process: regulation of translation; ribosome assembly; positive regulation of signal transduction; translational initiation; SREBP-mediated signaling pathway; protein amino acid phosphorylation

Research Articles on EIF2A

1. PERK/eIF2alpha signaling protects therapy resistant hypoxic cells through induction of glutathione synthesis and protection against ROS.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with EIF2A elisa kit

Reference Product	PubMed Publications
EIF5B elisa kit	>1 publications with EIF2A and EIF5B

Pathways associated with EIF2A elisa kit

Products by Pathway	Pathway Diagram
IL-6 Signaling Pathway antibodies	IL-6 Signaling Pathway Diagram
TGF-beta Receptor Signaling Pathway antibodies	TGF-beta Receptor Signaling Pathway Diagram

Diseases associated with EIF2A elisa kit

Disease Name	Pubmed Publications
Liver Diseases Antibodies	>4 publications with EIF2A and Liver Diseases
Gastrointestinal Diseases Antibodies	>2 publications with EIF2A and Gastrointestinal Diseases
Nervous System Diseases Antibodies	>2 publications with EIF2A and Nervous System Diseases
Atherosclerosis Antibodies	>1 publications with EIF2A and Atherosclerosis
Cell Transformation, Neoplastic Antibodies	>1 publications with EIF2A and Cell Transformation, Neoplastic
Fibrosis Antibodies	>1 publications with EIF2A and Fibrosis
Drug Toxicity Antibodies	>1 publications with EIF2A and Drug Toxicity
Neoplasms Antibodies	>1 publications with EIF2A and Neoplasms
Inflammation Antibodies	>1 publications with EIF2A and Inflammation
Reperfusion Injury Antibodies	>1 publications with EIF2A and Reperfusion Injury

Organs/Tissues associated with EIF2A elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>3 publications with EIF2A and Brain
Thyroid Antibodies	>2 publications with EIF2A and Thyroid
Lung Antibodies	>2 publications with EIF2A and Lung
Liver Antibodies	>2 publications with EIF2A and Liver
Muscle Antibodies	>2 publications with EIF2A and Muscle
Blood Antibodies	>1 publications with EIF2A and Blood
Intestine Antibodies	>1 publications with EIF2A and Intestine
Pancreas Antibodies	>1 publications with EIF2A and Pancreas
Kidney Antibodies	>1 publications with EIF2A and Kidney
Embryonic Tissue Antibodies	>1 publications with EIF2A and Embryonic Tissue