Monocyte Chemotactic Protein 3 (MCP-3) ELISA Kit for Horse

Name: Horse MCP-3 ELISA Kit; Monocyte Chemotactic Protein 3; MyBioSource
Brand: MyBioSource
SKU: MBS029950
Price: 675 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS029950

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated MCP-3 concentrations in Horse serum, plasma and other body fluids. Using Purified Horse MCP-3 antibody to coat Microelisa Stripplate… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Monocyte Chemotactic Protein 3 (MCP-3), ELISA Kit

Full Product Name

Horse Monocyte Chemotactic Protein 3 ELISA Kit

Product Gene Name

MCP-3 elisa kit

Species Reactivity

Horse

Assay Type

Sandwich

Samples

Serum, Plasma, Tissue Homogenate, Feces and Urine

Detection Range

6.25 pg/ml - 200 pg/ml.

Sensitivity

1.0 pg/ml.

Specificity

No significant cross-reactivity or interference between Horse MCP-3 and analogues was observed.

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Intra-assay Precision

Intra-assay CV (%) is less than 15%.

Molecular Weight

11,200 Da

OMIM

158106

3D Structure

ModBase 3D Structure for P80098

Intended Uses

This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated MCP-3 concentrations in Horse serum, plasma and other body fluids. Using Purified Horse MCP-3 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MCP-3 and MCP-3 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MCP-3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Other Notes

Small volumes of MCP-3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MCP-3 purchase

MBS029950 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Monocyte Chemotactic Protein 3 (MCP-3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MCP-3. The ELISA analytical biochemical technique of the MBS029950 kit is based on MCP-3 antibody-MCP-3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MCP-3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MCP-3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for MCP-3. It may not necessarily be applicable to this product.

NCBI GI #

313708

NCBI Accession #

CAA51055.1

UniProt Primary Accession #

P80098

UniProt Secondary Accession #

Q569J6

NCBI Official Full Name

monocyte chemotactic protein-3

UniProt Protein Name

C-C motif chemokine 7

UniProt Synonym Protein Names

Monocyte chemoattractant protein 3; Monocyte chemotactic protein 3; MCP-3; NC28; Small-inducible cytokine A7

Protein Family

C-C motif chemokine

UniProt Gene Name

CCL7

UniProt Synonym Gene Names

MCP3; SCYA6; SCYA7; MCP-3

UniProt Entry Name

CCL7_HUMAN

UniProt Comments for MCP-3

CCL7: Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q11.2-q12

Cellular Component: extracellular space; extracellular region

Molecular Function: heparin binding; CCR1 chemokine receptor binding; chemokine activity; CCR2 chemokine receptor binding

Biological Process: cellular calcium ion homeostasis; monocyte chemotaxis; regulation of cell shape; cell-cell signaling; eosinophil chemotaxis; response to gamma radiation; immune response; cytoskeleton organization and biogenesis; inflammatory response; chemotaxis; signal transduction; positive regulation of cell migration

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with MCP-3 elisa kit

Reference Product	PubMed Publications
CCL2 elisa kit	>221 publications with MCP-3 and CCL2
CCR2 elisa kit	>84 publications with MCP-3 and CCR2
CCL11 elisa kit	>68 publications with MCP-3 and CCL11
CCR3 elisa kit	>43 publications with MCP-3 and CCR3
CCR1 elisa kit	>40 publications with MCP-3 and CCR1
CCR5 elisa kit	>34 publications with MCP-3 and CCR5
CCL17 elisa kit	>22 publications with MCP-3 and CCL17
CCL22 elisa kit	>20 publications with MCP-3 and CCL22
CXCR3 elisa kit	>9 publications with MCP-3 and CXCR3
CCR10 elisa kit	>3 publications with MCP-3 and CCR10

Diseases associated with MCP-3 elisa kit

Disease Name	Pubmed Publications
Inflammation Antibodies	>159 publications with MCP-3 and Inflammation
Lung Diseases Antibodies	>53 publications with MCP-3 and Lung Diseases
Hypersensitivity Antibodies	>52 publications with MCP-3 and Hypersensitivity
Necrosis Antibodies	>45 publications with MCP-3 and Necrosis
Disease Models, Animal Antibodies	>41 publications with MCP-3 and Disease Models, Animal
Neoplasms Antibodies	>40 publications with MCP-3 and Neoplasms
Fibrosis Antibodies	>20 publications with MCP-3 and Fibrosis
Cardiovascular Diseases Antibodies	>18 publications with MCP-3 and Cardiovascular Diseases
Atherosclerosis Antibodies	>13 publications with MCP-3 and Atherosclerosis
Liver Diseases Antibodies	>10 publications with MCP-3 and Liver Diseases

Organs/Tissues associated with MCP-3 elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>246 publications with MCP-3 and Blood
Lung Antibodies	>64 publications with MCP-3 and Lung
Skin Antibodies	>29 publications with MCP-3 and Skin
Intestine Antibodies	>14 publications with MCP-3 and Intestine
Uterus Antibodies	>9 publications with MCP-3 and Uterus
Placenta Antibodies	>4 publications with MCP-3 and Placenta