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CD5 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3030194

Anti-Human CD5 can be used in research to study B-lymphocyte subsets or characterization of leukemias and lymphomas. More details

$275 / 1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD5, Monoclonal Antibody
Full Product Name
anti-Human CD5 antibody (UCHT-2)
Product Gene Name
Product Synonym Names
Leu-1
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
UCHT-2
Host
Mouse
Species Reactivity
Human
Form/Format
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Purity/Purification
>=95% monomer by analytical SEC, >95% by SDS Page
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Concentration
>=5.0mg/ml (lot specific)
Specificity
Mouse Anti-Human CD5 (Clone UCHT-2) recognizes Human CD5. Anti-Human CD5 recognizes a human T-lymphocyte surface membrane antigen (Mr 67 kDa).
Preparation and Storage
This antibody is stable for at least one week when stored sterile at 2-8 degree C. For long term storage aseptically aliquot in working volumes without diluting and store at -80 degree C. Avoid Repeated Freeze Thaw Cycles.
Matching Pairs
Molecular Weight
54,578 Da
Chromosome Location
Chromosome: 11; NC_000011.9 (60869930..60895324). Location: 11q13
OMIM
153340
3D Structure
ModBase 3D Structure for P06127
GMP
Manufactured according to cGMP (Current Good Manufacturing Practice) standards.
Endotoxin
<1.0 EU/mg as determined by the LAL method
Aggregation
Aggregation level <= 5%
Antigen Distribution
The CD5 antigen is present on approximately 70% of normal peripheral blood lymphocytes and >90% of all T-lymphocytes. T-lymphocytes in the thymus stain weakly while peripheral T-cells stain strongly. Monocytes, granulocytes and NK cells are not stained.1 Anti-CD5 also reacts with a distinct subset of normal B-lymphocytes, occasional cells in B-lymphocyte areas of spleen and lymph node, and most Ig+ B-chronic lymphoblastic leukemia (CLL) cells. The CD5 antigen has also been shown to be present on some lymphomas.

Product Categories/Family for anti-CD5 antibody

Applications Tested/Suitable for anti-CD5 antibody
CODEX (PhenoCycler-Fusion), Functional Assay (FUNC), Flow Cytometry (FC/FACS), Immunohistochemistry-Frozen (IHC-F)
Application Notes for anti-CD5 antibody
The suggested concentration for this CD5 antibody for staining cells in FC/FACS is <= 1.0 ug per 10^6 cells in a volume of 100 ul. Titration of the reagent is recommended for optimal performance for each application.

Other Notes

Small volumes of anti-CD5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD5 purchase

MBS3030194 is an antibody targeting CD5. Also known as anti-Human CD5 antibody (UCHT-2). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD5. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
T-cell surface glycoprotein CD5
NCBI Official Synonym Full Names
CD5 molecule
NCBI Official Symbol
CD5
NCBI Official Synonym Symbols
T1; LEU1
UniProt Protein Name
T-cell surface glycoprotein CD5
UniProt Synonym Protein Names
Lymphocyte antigen T1/Leu-1
UniProt Gene Name
CD5
UniProt Synonym Gene Names
LEU1
UniProt Entry Name
CD5_HUMAN
NCBI Protein Information
T-cell surface glycoprotein CD5; CD5 antigen (p56-62); lymphocyte antigen T1/Leu-1

UniProt Comments for CD5

CD5: a surface glycoprotein expressed on T cells and B-1 B cells. Rapidly recruited to the immunological synapse, lowering the response of the T cell antigen receptor. CD5 interacts with CD72/LYB-2. Promotes B-cell survival through stimulation of autocrine IL-10 production. Contains 3 SRCR domains.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: integral to plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; receptor activity; scavenger receptor activity

Biological Process: receptor-mediated endocytosis; cell proliferation; T cell costimulation; cell recognition
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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