Allopregnanolone/3a,5a-Tetrahydroprogesterone (AP/3A-5A-THP) ELISA Kit for Mouse

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated AP/3A-5A-THP concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse AP/3A-5A-THP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add AP/3A-5A-THP and AP/3A-5A-THP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of AP/3A-5A-THP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Small volumes of AP/3A-5A-THP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS030696 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Allopregnanolone/3A,5A-Tetrahydroprogesterone (AP/3A-5A-THP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AP/3A-5A-THP. The ELISA analytical biochemical technique of the MBS030696 kit is based on AP/3A-5A-THP antibody-AP/3A-5A-THP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AP/3A-5A-THP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AP/3A-5A-THP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).