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Taq 2X MeanGreen Master Mix Reagent

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS308024

Taq 2X MeanGreen™ MasterMix:Supplied as 2X reaction buffer with loading Dye dCTP, dGTP, dATP. dTTP. MgCI2 and Taq DNA polymerase Taq DNA Polymerase gene was isolated from Thermus aquaticus YT1 and expressed in E coli. Empirical's Taq 2X MeanGreenTM… More details

$305 / 250 Reactions +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Taq 2X MeanGreen Master Mix, Reagent
Full Product Name
Taq 2X MeanGreen Master Mix
Concentration
2X (lot specific)
Preparation and Storage
Upon receipt, store at -20°C. Room temperature for up to 60 days, 4°C for up to 3 months. Minimize freeze thaw of master mix to avoid loss of performance.
Protocol
The following reaction set up and general cycling conditions are recommended but can vary depending on the template and primers being used:
Component: Taq 2X MaxGreen MasterMix , Volume: 25ul , Final Concentration:1X ;
Component: Upstream Primer, 10uM , Volume: 0.5-5.0 ul , Final Concentration: 0.1-1.0um ;
Component: Downstream Primer, 10um ; Volume: 0.5-5.0 ul, Final Concentration: 0.1-1.0um ;
Component: DNA Template , Volume: Xul , Final Concentration: >1ng ;
Compound: Nuclease Free Water to Volume, Volume: 50ul , Final Concentration: N/A
Thermo Cycling Conditions
The following general cycling conditions are recommended but can vary depending on the template and primers being used:
Cycling Step: Initial Denaturation , Tempeture: 95°C, Holding Time: 2min, Cycles: 1
Cycling Step: Denaturation, Tempeture: 94-96°C, Holding Time: 15-30 sec, Cycles: 20-30
Cycling Step: Annealing#, Tempeture: 55-65°C, Holding Time:15-60 sec., Cycles: 20-30
Cycling Step: Extension, Temperature:70-72°C, Holding Time:1min/kb, Cycles: 20-30
Cycling Step: Final Extension, Temperature: 70-72°C, Holding Time: 0-10 min , Cycles: 1

#Annealing will depend on primer length and composition. Generally, begin 5°C below primer TM
Functionality
Taq 2x Mean GreenTM Master Mix is tested for performance in a polymerase chain reaction (PCR) using 50ul reaction volumes. Reactions containing the master mix, template DNA, and specific primers resulted in the expexted product from Human gDNA in comparison to a control lot of MasterMix.
Passing Result: Pass
Results: Pass
Unspecific dsEndonucleases
Taq 2X MeansGreenTMMaster Mix is tested for nuclease degradationin reactions containing a dsDNA or dsDNA substrate.
Passing Result: Pass
Results: Pass
E. coli Contamination
Taq 2X MeanGreenTM Master Mix is used as the template in a PCR reaction using primers specific to an E. coli gene No E.coli DNA is detected before 40 cycles.
Passing Result :Pass
Results: Pass
HdDNA Contamination
Taq 2X MeanGreenTM Master Mix is used as the temeplate in a PCR reaction using primers specific to Human gsDNA. No Human gDNA is detected before 40 cycles.
Passing Result: Pass
Result: Pass
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
ISO Certification
Manufactured in an ISO 13485:2016 Certified Laboratory.

Other Notes

Small volumes of Taq 2X MeanGreen Master Mix reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

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MBS308024 is a reagent targeting Taq 2X MeanGreen Master Mix.
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