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CD9 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS311967

MBS311967 is an antibody targeting CD9. Also known as MAb to CD9. Reactive in Human. Host: Mouse. Hybridization of NS/Ag 4.1 myeloma cells with spleen cells from BALB/c mice.Source: Ascites. More details

$820 / 0.2 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD9, Monoclonal Antibody
Full Product Name
MAb to CD9
Product Gene Name
anti-CD9 antibody
Product Synonym Names
Monoclonal Antibody to CD9 gp 24kD
Clonality
Monoclonal
Isotype
IgG1, kappa
Host
Host: Mouse. Hybridization of NS/Ag 4.1 myeloma cells with spleen cells from BALB/c mice.
Source: Ascites
Species Reactivity
Human
Form/Format
Purified, Lyophilized
Reconstitute with 1ml distilled water.
Concentration
0.2mg/ml (prior to lyophilization) (lot specific)
Specificity
CD9
The molecular weight of the recognized antigen is 24kDa. Recognizes 22-43% of peripheral non-T cells (B cells) (4). Recognizes blood platelets and a few granulocytic cells of normal bone marrow. Recognizes the distal convoluted tubule and the dista
Preparation and Storage
Store lyophilized product at 2 to 8 degree C. After reconstitution, aliquot and store at -20 degree C. Avoid multiple freeze/thaw cycles. The addition of 0.1% (w/v) sodium azide is recommended for storage up to one month at 2 to 8 degree C.
Molecular Weight
25,416 Da
Chromosome Location
Chromosome: 12; NC_000012.11 (6309555..6347427). Location: 12p13.3
OMIM
143030
3D Structure
ModBase 3D Structure for P21926
Immunogen
Human bone marrow malignant cells from acute non-T, non-B leukemia
Affinity Constant
Not determined
Buffer
Lyophilized from PBS containing 1mg/ml BSA
Preservative
No
Lyophilized
Yes
Important Note
Centrifuge before opening to ensure complete recovery of vial contents.

Product Categories/Family for anti-CD9 antibody

Monoclonal Antibodies to Human CDs
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-CD9 antibody
Flow Cytometry, Immunofluorescence Assay, Immunohistochemistry - Formalin
Application Notes for anti-CD9 antibody
Fluorescent microscopy or flow cytometry: 2ug/5x105 cells/test
Immunohistochemistry: Working dilution 1:25 to 1:50. Suitable on frozen sections or cell smears.
Each laboratory should determine an optimum working titer for use in its particular appli

Product References and Citations for anti-CD9 antibody

• Boucheix, C., et.al., (1983), "Characteristics of platelet aggregation induced by the monoclonal antibody ALB6 (acute lymphoblastic leukemia antigen p24)", FEBS - Letters, 161: 289-294.
• Boucheix, C., et al., (1988), "CD9 antigen

Other Notes

Small volumes of anti-CD9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD9. It may not necessarily be applicable to this product.

NCBI GI #
4502693
NCBI GeneID
928
NCBI Accession #
NP_001760.1
UniProt Primary Accession #
P21926
UniProt Secondary Accession #
Q5J7W6; Q96ES4; D3DUQ9
UniProt Related Accession #
P21926; Q56CY1
GenBank Nucleotide #
NM_001769.3
NCBI Official Full Name
CD9 antigen
NCBI Official Synonym Full Names
CD9 molecule
NCBI Official Symbol
CD9
NCBI Official Synonym Symbols
MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; FLJ99568; TSPAN-29
UniProt Protein Name
CD9 antigen
UniProt Synonym Protein Names
5H9 antigen; Cell growth-inhibiting gene 2 protein; Leukocyte antigen MIC3; Motility-related protein; MRP-1; Tetraspanin-29; Tspan-29; p24
Protein Family
CD9 antigen
UniProt Gene Name
CD9
UniProt Synonym Gene Names
MIC3; TSPAN29
UniProt Entry Name
CD9_HUMAN
NCBI Protein Information
CD9 antigen; 5H9 antigen; tetraspanin-29; BA-2/p24 antigen; CD9 antigen (p24); OTTHUMP00000041574; OTTHUMP00000041575; OTTHUMP00000041576; OTTHUMP00000238624; leukocyte antigen MIC3; motility related protein-1; cell growth-inhibiting gene 2 protein

NCBI Summary for CD9

This gene encodes a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis. [provided by RefSeq]
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UniProt Comments for CD9

Function: Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion. Ref.11 Ref.12 Ref.13

Subunit structure: Forms both disulfide-linked homodimers and higher homooligomers as well as heterooligomers with other members of the tetraspanin family. Associates with CR2/CD21 and with PTGFRN/CD9P1. Interacts directly with IGSF8. Ref.14 Ref.16 Ref.17

Subcellular location: Membrane; Multi-pass membrane protein.

Tissue specificity: Expressed by a variety of hematopoietic and epithelial cells.

Post-translational modification: Protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids.

Sequence similarities: Belongs to the tetraspanin (TM4SF) family.
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Research Articles on CD9

1. Functional and biochemical studies of CD9 in fibrosarcoma cell line.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD9 antibody

 Reference Product  PubMed Publications
 CD81 antibody  >172 publications with CD9 and CD81

Pathways associated with anti-CD9 antibody

 Products by Pathway  Pathway Diagram
 Formation Of Platelet Plug Pathway antibodies  Formation Of Platelet Plug Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Platelet Activation Pathway antibodies  Platelet Activation Pathway Diagram
 Platelet Degranulation Pathway antibodies  Platelet Degranulation Pathway Diagram
 Response To Elevated Platelet Cytosolic Ca2+ Pathway antibodies  Response To Elevated Platelet Cytosolic Ca2+ Pathway Diagram
 A6b1 And A6b4 Integrin Signaling Pathway antibodies  A6b1 And A6b4 Integrin Signaling Pathway Diagram

Diseases associated with anti-CD9 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >351 publications with CD9 and Neoplasms
 Leukemia Antibodies  >150 publications with CD9 and Leukemia
 Lymphoma Antibodies  >66 publications with CD9 and Lymphoma
 Leukemia, Myeloid Antibodies  >55 publications with CD9 and Leukemia, Myeloid
 Neoplasm Metastasis Antibodies  >52 publications with CD9 and Neoplasm Metastasis
 Neoplasms, Germ Cell and Embryonal Antibodies  >49 publications with CD9 and Neoplasms, Germ Cell and Embryonal
 Leukemia, Myeloid, Acute Antibodies  >45 publications with CD9 and Leukemia, Myeloid, Acute
 Lung Diseases Antibodies  >43 publications with CD9 and Lung Diseases
 Male Urogenital Diseases Antibodies  >41 publications with CD9 and Male Urogenital Diseases
 Cardiovascular Diseases Antibodies  >38 publications with CD9 and Cardiovascular Diseases

Organs/Tissues associated with anti-CD9 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >64 publications with CD9 and Lung
 Embryonic Tissue Antibodies  >51 publications with CD9 and Embryonic Tissue
 Uterus Antibodies  >24 publications with CD9 and Uterus
 Brain Antibodies  >23 publications with CD9 and Brain
 Prostate Antibodies  >15 publications with CD9 and Prostate
 Mixed Antibodies  >11 publications with CD9 and Mixed
 Intestine Antibodies  >10 publications with CD9 and Intestine
 Pharynx Antibodies  >9 publications with CD9 and Pharynx
 Mouth Antibodies  >8 publications with CD9 and Mouth
 Pancreas Antibodies  >6 publications with CD9 and Pancreas
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