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MAP-2 (2a + 2b) Microtubule Associated Protein 2 (MAP2) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS312164

MBS312164 is an antibody targeting MAP2. Also known as MAb to MAP-2 (2a + 2b). Host: MouseSource: Cell Culture. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Technical Datasheet View PDF
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Product Overview

Product Name
MAP-2 (2a + 2b) Microtubule Associated Protein 2 (MAP2), Monoclonal Antibody
Full Product Name
MAb to MAP-2 (2a + 2b)
Product Gene Name
Product Synonym Names
Monoclonal Antibody to Microtubule Associated Protein-2 (MAP-2) (2a + 2b)
Clonality
Monoclonal
Isotype
IgG1
Host
Host: Mouse
Source: Cell Culture
Form/Format
Biotin, Liquid
Purity/Purification
Protein A chromatography
Concentration
100ug/ml (OD280nm) (lot specific)
Specificity
MAP-2 (2a + 2b) Microtubule Associated Protein 2
Recognizes (Mr 300kDa) MAP-2 protein from bovine brain. The antibody strongly cross-reacts with MAP-2 from human, rat, mouse and chicken brains. Anti-MAP-2 (Clone AP20) reacts with the high molecular wei
Preparation and Storage
Store at 2 to 8 degree C. DO NOT FREEZE!
Molecular Weight
199,526 Da
Chromosome Location
Chromosome: 2; NC_000002.11 (210288771..210598834). Location: 2q34-q35
OMIM
157130
3D Structure
ModBase 3D Structure for P11137
Affinity Constant
Not determined
Buffer
0.01M PBS, pH 7.4 containing 1% BSA
Preservative
0.09% Sodium azide
Important Note
Centrifuge before opening to ensure complete recovery of vial contents.
Warnings
This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1 - 1.0 %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-MAP2 antibody
Immunohistochemistry - Formalin, Western Blot
Application Notes for anti-MAP2 antibody
Can be used in protein blotting and histology, frozen tissues only. Anti-MAP-2 may be used in studies of neuron structure. The suggested working dilution for immunohistochemical staining is approximately 1:500. Each laboratory should determine an optimum

Product References and Citations for anti-MAP2 antibody

• Cacares, A. et al (1984), J. Neuroscience, 4:394.
• Peng, I. et al (1986), J. Cell Biol., 102:252.
• Lewis, S.A. et al (1989), Nature, 342:498.

Other Notes

Small volumes of anti-MAP2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MAP2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Microtubule-associated protein 2
NCBI Official Synonym Full Names
microtubule-associated protein 2
NCBI Official Symbol
MAP2
NCBI Official Synonym Symbols
MAP2A; MAP2B; MAP2C; DKFZp686I2148
UniProt Protein Name
Microtubule-associated protein 2
UniProt Gene Name
MAP2
UniProt Entry Name
MAP2_HUMAN
NCBI Protein Information
microtubule-associated protein 2; MAP-2; OTTHUMP00000163915; OTTHUMP00000163916; OTTHUMP00000165185; OTTHUMP00000208143; OTTHUMP00000208144

NCBI Summary for MAP2

This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq]
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UniProt Comments for MAP2

Function: The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.

Subcellular location: Cytoplasm › cytoskeleton

Probable.

Post-translational modification: MAP2A/c is phosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.9 Ref.10 Ref.11 Ref.12

Sequence similarities: Contains 3 Tau/MAP repeats.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-MAP2 antibody

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