Amyloid Beta 1-42 (ABETA42) Antibody – Mouse Monoclonal

mmunoblotting, Immunohistochemistry: Im m unofluorescence, Immunoprecip i tation Test at 1-10ug/ml. Sandwich ELISA (as capture antibody with #MBS190935 as HRP - c onjugated detection antibody). These are recommended concentrations; enduser should determine optimal concentrations for their applications. Sandwich ELISA protoco l on next page. See specific product references below for more information.

Sandwich ELISA Protocol:
96-well ELISA plates are coated with capture MAbat 2.5-5ug/well and incubated at 4 degree C overnight. The next day 300ul of blocking buffer is added, and plates are again incubated at 4 degree C overnight. The next day plates are washed in PBS and serial dilution of A beta samples are added; plates are incubated overnight at 4 degree C. The next day plates are washed in PBS, and HRP-conjugated detection Abeta MAb is added; plates are incubated for 4hrs at room temperature. Plates are washed in PBS-Tween and developed with TMB substrate for 5 mins