DARPP-32 Antibody – Rabbit

This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

DARPP-32: a member of the protein phosphatase inhibitor 1 family. A dopamine- and cyclic AMP-regulated neuronal phosphoprotein. Both dopaminergic and glutamatergic (NMDA) receptor stimulation regulate the extent of DARPP32 phosphorylation, but in opposite directions. Dopamine D1 receptor stimulation enhances cAMP formation, resulting in the phosphorylation of DARPP32; phosphorylated DARPP32 is a potent protein phosphatase-1 inhibitor. NMDA receptor stimulation elevates intracellular calcium, which leads to activation of calcineurin and dephosphorylation of phospho-DARPP32, thereby reducing the phosphatase-1 inhibitory activity of DARPP32. Two alternatively-spliced isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Protein phosphatase, regulatory subunit

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: cell soma; cytoplasm; nucleus; cytosol

Molecular Function: protein kinase inhibitor activity; protein phosphatase type 1 regulator activity; protein phosphatase inhibitor activity; D4 dopamine receptor binding; D3 dopamine receptor binding; D1 dopamine receptor binding; type 1 serine/threonine specific protein phosphatase inhibitor activity; D2 dopamine receptor binding; D5 dopamine receptor binding

Biological Process: negative regulation of female receptivity; transcription, DNA-dependent; regulation of catalytic activity; response to amphetamine; negative regulation of catalytic activity; visual learning; negative regulation of protein kinase activity; signal transduction