Glypican 2 (GPC2) ELISA Kit for Rat

Name: Rat GPC2 ELISA Kit; Glypican 2; MyBioSource
Brand: MyBioSource
SKU: MBS031470
Price: 675 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS031470

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated GPC2 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat GPC2 antibody to coat Microelisa… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Glypican 2 (GPC2), ELISA Kit

Full Product Name

Rat Glypican 2 ELISA Kit

Product Gene Name

GPC2 elisa kit

Species Reactivity

Rat

Assay Type

Sandwich

Samples

Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids

Detection Range

62.5 pg/ml - 2000 pg/ml.

Sensitivity

10 pg/ml.

Specificity

No significant cross-reactivity or interference between Rat GPC2 and analogues was observed.

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]

Intra-assay Precision

Intra-assay CV (%) is less than 15%.

Molecular Weight

62,830 Da

Chromosome Location

Chromosome: 7; NC_000007.13 (99767229..99774990, complement). Location: 7q22.1

3D Structure

ModBase 3D Structure for Q8N158

Intended Uses

This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated GPC2 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat GPC2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GPC2 and GPC2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GPC2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Other Notes

Small volumes of GPC2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GPC2 purchase

MBS031470 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Glypican 2 (GPC2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GPC2. The ELISA analytical biochemical technique of the MBS031470 kit is based on GPC2 antibody-GPC2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GPC2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GPC2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for GPC2. It may not necessarily be applicable to this product.

NCBI GI #

22749459

NCBI GeneID

221914

NCBI Accession #

NP_689955.1

UniProt Primary Accession #

Q8N158

UniProt Secondary Accession #

A4D2A7

UniProt Related Accession #

Q8N158

GenBank Nucleotide #

NM_152742.1

NCBI Official Full Name

glypican-2

NCBI Official Synonym Full Names

glypican 2

NCBI Official Symbol

GPC2

UniProt Protein Name

Glypican-2

Protein Family

Glypican

UniProt Gene Name

GPC2

UniProt Entry Name

GPC2_HUMAN

NCBI Protein Information

glypican-2; cerebroglycan; glypican proteoglycan 2, cerebroglycan proteoglycan

UniProt Comments for GPC2

GPC2: Cell surface proteoglycan that bears heparan sulfate. May fulfill a function related to the motile behaviors of developing neurons. Belongs to the glypican family.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: lysosomal lumen; proteinaceous extracellular matrix; extracellular space; endoplasmic reticulum; Golgi lumen; plasma membrane

Molecular Function: heparan sulfate proteoglycan binding

Biological Process: neuron differentiation; chondroitin sulfate metabolic process; phototransduction, visible light; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; smoothened signaling pathway; glycosaminoglycan metabolic process; carbohydrate metabolic process; pathogenesis; retinoid metabolic process

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GPC2 elisa kit

Reference Product	PubMed Publications
GPC3 elisa kit	>10 publications with GPC2 and GPC3
SDC4 elisa kit	>1 publications with GPC2 and SDC4
SDC1 elisa kit	>1 publications with GPC2 and SDC1

Pathways associated with GPC2 elisa kit

Products by Pathway	Pathway Diagram
A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway antibodies	A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway Diagram
Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway antibodies	Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
Diseases Associated With Visual Transduction Pathway antibodies	Diseases Associated With Visual Transduction Pathway Diagram
Glycosaminoglycan Metabolism Pathway antibodies	Glycosaminoglycan Metabolism Pathway Diagram
Glypican 2 Network Pathway antibodies	Glypican 2 Network Pathway Diagram
Glypican Pathway antibodies	Glypican Pathway Diagram
HS-GAG Biosynthesis Pathway antibodies	HS-GAG Biosynthesis Pathway Diagram
HS-GAG Degradation Pathway antibodies	HS-GAG Degradation Pathway Diagram
Heparan Sulfate/heparin (HS-GAG) Metabolism Pathway antibodies	Heparan Sulfate/heparin (HS-GAG) Metabolism Pathway Diagram

Diseases associated with GPC2 elisa kit

Disease Name	Pubmed Publications
Edema Antibodies	>1 publications with GPC2 and Edema

Organs/Tissues associated with GPC2 elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>3 publications with GPC2 and Brain
Embryonic Tissue Antibodies	>1 publications with GPC2 and Embryonic Tissue