Renin (REN) Antibody – Sheep Polyclonal

Renin catalyzes the first step in the activation pathway of angiotensinogen--a cascade that can result in aldosterone release,vasoconstriction, and increase in blood pressure. Renin, an aspartyl protease, cleaves angiotensinogen to form angiotensin I, which is converted to angiotensin II by angiotensin I converting enzyme, an important regulator of blood pressure and electrolyte balance. Transcript variants that encode different protein isoforms and that arise from alternative splicing and the use of alternative promoters have been described, but their full-length nature has not been determined. Mutations in this gene have been shown to cause familial hyperproreninemia. [provided by RefSeq]

Function: Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.

Catalytic activity: Cleavage of Leu-|-Xaa bond in angiotensinogen to generate angiotensin I.

Enzyme regulation: Interaction with ATP6AP2 results in a 5-fold increased efficiency in angiotensinogen processing.

Subunit structure: Interacts with ATP6AP2. Ref.14

Subcellular location: Secreted. Membrane. Note: Associated to membranes via binding to ATP6AP2.

Involvement in disease: Defects in REN are a cause of renal tubular dysgenesis (RTD) [

MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Ref.17Defects in REN are the cause of familial juvenile hyperuricemic nephropathy type 2 (HNFJ2) [

MIM:613092]. It is a renal disease characterized by juvenile onset of hyperuricemia, slowly progressive renal failure and anemia. Ref.18

Sequence similarities: Belongs to the peptidase A1 family.

Biophysicochemical propertiesKinetic parameters:KM=1 µM for angiotensinogen (in absence of ATP6AP2)KM=0.15 µM for angiotensinogen (in presence of membrane-bound ATP6AP2)