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IgG, F(ab')2 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS316487

MBS316487 is an antibody targeting IgG, F(ab')2. Also known as Goat anti Human IgG F (ab')2. Host: Goat. More details

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Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
IgG, F(ab')2, Polyclonal Antibody
Full Product Name
Goat anti Human IgG F (ab')2
Product Synonym Names
Goat anti Human IgG, F (ab') 2; Goat Antibody to Human Immunoglobulin G (IgG), F(ab') 2 Fragment specific
Clonality
Polyclonal
Host
Goat
Form/Format
HRP, Lyophilized
Reconstitute to 2.0 ml with distilled water. Centrifuge product if it is not completely clear after standing for 1-2 hour at room temperature. To judge clarity, draw product into a Pasteur pipette.
Purity/Purification
Isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Concentration
0.8mg/ml (prior to lyophilization) (lot specific)
Specificity
IgG, F(ab')2
Based on immunoelectrophoresis, the antibody reacts with the light chains on human IgG and with those common to other human immunoglobulins. Does not react with the Fc portion of human IgG. No antibody was detected against non-immunoglobul
Preparation and Storage
Store lyophilized product at 2 to 8 degree C. After reconstitution, product is stable for several weeks at 2 to 8 degree C as an undiluted liquid. For long term storage, aliquot and store at -20 degree C. For extended storage, add an equal volume of glyce
Buffer
Lyophilized from 0.01M Sodium Phosphate, 0.25M Sodium Chloride, pH 7.6 containing 15mg/ml BSA
Preservative
No
Lyophilized
Yes
Warnings
Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.

Product Categories/Family for anti-IgG, F(ab')2 antibody

Polyclonal Antibodies to Human Immunoglobulins
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Application Notes for anti-IgG, F(ab')2 antibody
A dilution range of 1:500 - 1:5,000 is suggested for immunohistochemistry on tissue sections, 1:5,000 - 1:100,000 for ELISA and Western blotting with chromogenic substrates and 1:10,000 - 1:200,000 for Western blotting with ECL substrates. Each laboratory

Other Notes

Small volumes of anti-IgG, F(ab')2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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