ELISA ULTRABLOCK Accessory Reagent

Ready To Use
Ready to use - liquid

Store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted.
Shelf Life: 18 months from date of despatch.

0.05% Sodium Azide
Intended Use||This item is a high performance ELISA blocking buffer for use in all ELISA formats with high background problems. The formulation includes a proprietary fish extract, molecular stabilizers and an antimicrobial agent. It is particularly useful in antigen-down and sandwich ELISA assays with mammalian samples, especially human, bovine and porcine serum, where the non-mammalian blocking proteins are less likely to interact with endogenous antibodies in the serum sample.
Superior blocking is possible due to the small size of the molecules which block non-specific binding sites on the adsorbed protein and unoccupied regions of the polystyrene plates often sterically inaccessible to traditional blockers. It provides a long-term stable environment for coating antigen or capture antibody. Plates can be blocked at room temperature and stored once dried for up to a year at 4 degree C.
Instructions For Use||1. Coat ELISA plate with antibody or antigen as required. 2. After incubation, remove the coating solution and wash the plate x2 with wash buffer. 3. Add 300-400ul of This item and incubate for 2-24 hours. Use a volume equal to or greater than the volume of coating solution. 4. After removal of the blocking buffer continue with the assay or dry the plate for long-term storage at 4 degree C.

Phosphate buffered saline