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GTF2F2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3200435

This is a rabbit polyclonal antibody against GTF2F2. It was validated on Western Blot and immunohistochemistryTarget Description: GTF2F2 synergizes with HIV-1 Tat and the cellular coactivator Tat-SF1 during Tat-mediated transactivation of the HIV-1 LTR… More details

$440 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GTF2F2, Polyclonal Antibody
Full Product Name
GTF2F2 antibody - middle region
Product Gene Name
anti-GTF2F2 antibody
Product Synonym Gene Name
BTF4; RAP30; TF2F2; TFIIF
Immunogen Sequence
Synthetic peptide located within the following region: IEESSKPVRL SQQLDKVVTT NYKPVANHQY NIEYERKKKE DGKRARADKQ
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Purity/Purification
Protein A purified
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
GTF2F2 peptide (MBS3225489) is used for blocking the activity of GTF2F2 antibody (MBS3200435)
Molecular Weight
28kDa
OMIM
189969
3D Structure
ModBase 3D Structure for P13984
Homology
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 77%
Immunogen
The immunogen is a synthetic peptide directed towards the middle region of human GTF2F2

Product Categories/Family for anti-GTF2F2 antibody

Polyclonal; Transcription Factor; Transcription Regulation; Transcription Regulation; Immunohistochemistry; Transcription Factors
Applications Tested/Suitable for anti-GTF2F2 antibody
Immunohistochemistry (IHC), Western Blot (WB)

Immunohistochemistry (IHC) of anti-GTF2F2 antibody

Rabbit Anti-GTF2F2 Antibody
Paraffin Embedded Tissue: Human Brain
Cellular Data: Neural Cells
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X
Rabbit Anti-GTF2F2 Polyclonal Antibody – Cat# MBS3200435

Western Blot (WB) of anti-GTF2F2 antibody

Host: Mouse
Target Name: GTF2F2
Sample Tissue: Mouse Small Intestine
Antibody Dilution: 1ug/ml
Rabbit Anti-GTF2F2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3200435

Western Blot (WB) of anti-GTF2F2 antibody

WB Suggested Anti-GTF2F2 Antibody Titration: 1.0ug/ml
Positive Control: HepG2 cell lysate
Rabbit Anti-GTF2F2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3200435

Other Notes

Small volumes of anti-GTF2F2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GTF2F2 purchase

MBS3200435 is an antibody targeting GTF2F2. Also known as GTF2F2 antibody - middle region. Reactive in Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GTF2F2. It may not necessarily be applicable to this product.

NCBI GI #
4758488
NCBI GeneID
2963
NCBI Accession #
NP_004119
UniProt Primary Accession #
P13984
UniProt Related Accession #
P13984
GenBank Nucleotide #
NM_004128
NCBI Official Full Name
general transcription factor IIF subunit 2
NCBI Official Synonym Full Names
general transcription factor IIF subunit 2
NCBI Official Symbol
GTF2F2
NCBI Official Synonym Symbols
BTF4; RAP30; TF2F2; TFIIF
UniProt Protein Name
General transcription factor IIF subunit 2
UniProt Synonym Protein Names
ATP-dependent helicase GTF2F2; General transcription factor IIF 30 kDa subunit; Transcription initiation factor IIF subunit beta; TFIIF-beta; Transcription initiation factor RAP30
Protein Family
General transcription factor
UniProt Gene Name
GTF2F2
UniProt Synonym Gene Names
RAP30; TFIIF-beta
UniProt Entry Name
T2FB_HUMAN
NCBI Protein Information
general transcription factor IIF subunit 2

UniProt Comments for GTF2F2

GTF2F2: TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. This subunit shows ATP-dependent DNA- helicase activity. Belongs to the TFIIF beta subunit family.

Protein type: Transcription initiation complex; Nuclear receptor co-regulator; EC 3.6.4.12; Helicase

Chromosomal Location of Human Ortholog: 13q14

Cellular Component: nucleoplasm; microtubule cytoskeleton; transcription factor TFIIF complex; nucleus

Molecular Function: protein binding; DNA binding; ATP-dependent helicase activity; ATP binding

Biological Process: nuclear mRNA splicing, via spliceosome; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; mRNA capping; regulation of transcription, DNA-dependent; viral reproduction; positive regulation of viral transcription; RNA splicing; RNA elongation from RNA polymerase II promoter; gene expression
Show more

Research Articles on GTF2F2

1. Data show that TFIIF has an important role in stabilizing TFIIB within the PIC and after transcription initiates.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GTF2F2 antibody

 Reference Product  PubMed Publications
 GTF2F1 antibody  >2 publications with GTF2F2 and GTF2F1
 GTF2B antibody  >1 publications with GTF2F2 and GTF2B

Pathways associated with anti-GTF2F2 antibody

 Products by Pathway  Pathway Diagram
 Abortive Elongation Of HIV-1 Transcript In The Absence Of Tat Pathway antibodies  Abortive Elongation Of HIV-1 Transcript In The Absence Of Tat Pathway Diagram
 Basal Transcription Factors Pathway antibodies  Basal Transcription Factors Pathway Diagram
 Basal Transcription Factors Pathway antibodies  Basal Transcription Factors Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Eukaryotic Transcription Initiation Pathway antibodies  Eukaryotic Transcription Initiation Pathway Diagram
 Formation Of HIV Elongation Complex In The Absence Of HIV Tat Pathway antibodies  Formation Of HIV Elongation Complex In The Absence Of HIV Tat Pathway Diagram
 Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway antibodies  Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway Diagram
 Formation Of RNA Pol II Elongation Complex Pathway antibodies  Formation Of RNA Pol II Elongation Complex Pathway Diagram
 Formation Of The Early Elongation Complex Pathway antibodies  Formation Of The Early Elongation Complex Pathway Diagram
 Formation Of The HIV-1 Early Elongation Complex Pathway antibodies  Formation Of The HIV-1 Early Elongation Complex Pathway Diagram

Organs/Tissues associated with anti-GTF2F2 antibody

 Organ/Tissue Name  Pubmed Publications
 Mammary Gland Antibodies  >1 publications with GTF2F2 and Mammary Gland
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