Rabbit Anti-TLE3 Polyclonal Antibody – Cat# MBS3200886

WB Suggested Anti-TLE3 Antibody Titration: 2.5ug/ml
Positive Control: HepG2 cell lysate

TLE3 Antibody – Rabbit Polyclonal

Name: TLE3 Antibody; Polyclonal; Rabbit; MyBioSource
Brand: MyBioSource
SKU: MBS3200886
Price: 440 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3200886

This is a rabbit polyclonal antibody against TLE3. It was validated on Western Blot using a cell lysate as a positive control.Target Description: The TLE genes are differentially expressed and encode nuclear proteins, consistent with the presence of sequence… More details

$440 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

TLE3, Polyclonal Antibody

Full Product Name

TLE3 antibody - N-terminal region

Product Gene Name

anti-TLE3 antibody

Product Synonym Gene Name

ESG; ESG3; GRG3; HsT18976

Immunogen Sequence

Synthetic peptide located within the following region: GSSSGLLALG ALGSQAHLTV KDEKNHHELD HRERESSANN SVSPSESLRA

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat, Zebrafish

Form/Format

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Purity/Purification

Protein A purified

Preparation and Storage

For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Antibody/Peptide Pairs

TLE3 peptide (MBS3225923) is used for blocking the activity of TLE3 antibody (MBS3200886)

Molecular Weight

83kDa

OMIM

600190

3D Structure

ModBase 3D Structure for Q04726

Homology

Cow: 100%; Dog: 100%; Guinea Pig: 93%; Horse: 93%; Human: 100%; Mouse: 93%; Pig: 100%; Rabbit: 93%; Rat: 93%; Zebrafish: 79%

Immunogen

The immunogen is a synthetic peptide directed towards the N terminal region of human TLE3

Product Categories/Family for anti-TLE3 antibody

Polyclonal; Transcription Factor; Transcription Regulation; Chromatin & Nuclear Signaling; Signal Transduction; Transcription Factors

Applications Tested/Suitable for anti-TLE3 antibody

Western Blot (WB)

Other Notes

Small volumes of anti-TLE3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TLE3 purchase

MBS3200886 is an antibody targeting TLE3. Also known as TLE3 antibody - N-terminal region. Reactive in Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat, Zebrafish. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TLE3. It may not necessarily be applicable to this product.

NCBI GI #

157384982

NCBI GeneID

7090

NCBI Accession #

NP_005069

UniProt Primary Accession #

Q04726

UniProt Related Accession #

Q04726

GenBank Nucleotide #

NM_005078

NCBI Official Full Name

transducin-like enhancer protein 3 isoform a

NCBI Official Synonym Full Names

TLE family member 3, transcriptional corepressor

NCBI Official Symbol

TLE3

NCBI Official Synonym Symbols

ESG; ESG3; GRG3; HsT18976

UniProt Protein Name

Transducin-like enhancer protein 3

UniProt Synonym Protein Names

Enhancer of split groucho-like protein 3; ESG3

Protein Family

Transducin-like enhancer protein

UniProt Gene Name

TLE3

UniProt Synonym Gene Names

KIAA1547; ESG3

UniProt Entry Name

TLE3_HUMAN

NCBI Protein Information

transducin-like enhancer protein 3

NCBI Summary for TLE3

This gene encodes a transcriptional co-repressor protein that belongs to the transducin-like enhancer family of proteins. The members of this family function in the Notch signaling pathway that regulates determination of cell fate during development. Expression of this gene has been associated with a favorable outcome to chemotherapy with taxanes for ovarian carcinoma. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Sep 2013]

UniProt Comments for TLE3

TLE3: transcriptional corepressor that binds to a number of transcription factors. Belongs to the WD-repeat Groucho/TLE family. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Four alternatively spliced isoforms have been described.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 15q22

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding

Biological Process: Notch signaling pathway; organ morphogenesis; Wnt receptor signaling pathway; regulation of transcription, DNA-dependent; transcription, DNA-dependent; signal transduction

Research Articles on TLE3

1. Patients with TLE3-negative tumors displayed poorer outcomes regarding progression-free survival than patients with TLE3-positive tumors when prognosis within the group of patients with triple-negative breast cancer (TNBC) lesions was analyzed. In contrast, no such difference in prognosis was found in a comparison of TLE-3 positive/negative patients in the group of all patients.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.