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HES1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3200894

This is a rabbit polyclonal antibody against HES1. It was validated on Western Blot and immunohistochemistryTarget Description: HES1 belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require… More details

$440 / 0.1 mL +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
HES1, Polyclonal Antibody
Full Product Name
HES1 antibody - N-terminal region
Product Gene Name
anti-HES1 antibody
Product Synonym Gene Name
HHL; HRY; HES-1; bHLHb39
Immunogen Sequence
Synthetic peptide located within the following region: NTTPDKPKTA SEHRKSSKPI MEKRRRARIN ESLSQLKTLI LDALKKDSSR
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Tested Species Reactivity: Human Mouse
Predicted Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Zebrafish
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Purity/Purification
Protein A purified
Concentration
1.0 mg/mL (lot specific)
Preparation and Storage
For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
HES1 peptide (MBS3225931) is used for blocking the activity of HES1 antibody (MBS3200894)
Molecular Weight
30kDa
OMIM
139605
3D Structure
ModBase 3D Structure for Q14469
Protein Size (#AA)
280 amino acids
Homology
Cow: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of human HES1
Protein Interactions
STAT3; JAK2; APH1A; LTBR; ASGR2; TLE1; ELAVL1; PARP1; UBC; NUDT3; HDAC6; YWHAB; PTK2; NR4A1; HMGCL; GAPDH; FHL1; CSNK1E; APCS; FANCE; FANCC; FANCA; FANCL; SPTAN1; HSP90AA1; HSPA4; FANCG; FANCF; HDAC1; RUNX2; HES6; ID4; ID1; SIRT1; UBQLN1; HEY2; ID2; ID3;
Sample Type Confirmation
HES1 is strongly supported by BioGPS gene expression data to be expressed in Raji

Product Categories/Family for anti-HES1 antibody

Polyclonal; Transcription Factor; Neurobiology; Transcription Regulation; Phosphorylation; Transcription Factors; Cell Morphogenesis; Cell Differentiation
Applications Tested/Suitable for anti-HES1 antibody
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blot (WB)

Immunofluorescence (IF) of anti-HES1 antibody

transfected Ls174T cell lysate at 50ug/ml.
Rabbit Anti-HES1 Polyclonal Antibody – Cat# MBS3200894

Immunohistochemistry (IHC) of anti-HES1 antibody

Rabbit Anti-HES1 Antibody
Paraffin Embedded Tissue: Human cardiac cell
Cellular Data: Epithelial cells of renal tubule
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X
Rabbit Anti-HES1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS3200894

Immunoprecipitation (IP) of anti-HES1 antibody

K562
Rabbit Anti-HES1 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS3200894

Western Blot (WB) of anti-HES1 antibody

HES1 antibody - N-terminal region validated by WB using Mouse embryo tissue at 1:800.
Rabbit Anti-HES1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3200894

Western Blot (WB) of anti-HES1 antibody

WB Suggested Anti-HES1 Antibody Titration: 1.25ug/ml
Positive Control: Raji cell lysateHES1 is strongly supported by BioGPS gene expression data to be expressed in Human Raji cells
Rabbit Anti-HES1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3200894

Western Blot (WB) of anti-HES1 antibody

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. Canonical 30 kDa isoform is identified, and a second isoform of 23 kDa is also present in some samples in N36.
Rabbit Anti-HES1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3200894

Western Blot (WB) of anti-HES1 antibody

HES1 antibody - N-terminal region validated by WB using transfected Ls174Tcell lysate at 1ug/ml.
Rabbit Anti-HES1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3200894

Other Notes

Small volumes of anti-HES1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HES1 purchase

MBS3200894 is an antibody targeting HES1. Also known as HES1 antibody - N-terminal region. Reactive in Tested Species Reactivity: Human Mouse
Predicted Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Zebrafish. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for HES1. It may not necessarily be applicable to this product.

NCBI GI #
5031763
NCBI GeneID
3280
NCBI Accession #
NP_005515
UniProt Primary Accession #
Q14469
UniProt Related Accession #
Q14469
GenBank Nucleotide #
NM_005524
NCBI Official Full Name
transcription factor HES-1
NCBI Official Synonym Full Names
hes family bHLH transcription factor 1
NCBI Official Symbol
HES1
NCBI Official Synonym Symbols
HHL; HRY; HES-1; bHLHb39
UniProt Protein Name
Transcription factor HES-1
UniProt Synonym Protein Names
Class B basic helix-loop-helix protein 39; bHLHb39; Hairy and enhancer of split 1; Hairy homolog; Hairy-like protein
Protein Family
Protein
UniProt Gene Name
HES1
UniProt Synonym Gene Names
BHLHB39; HL; HRY; bHLHb39; hHL
UniProt Entry Name
HES1_HUMAN
NCBI Protein Information
transcription factor HES-1

NCBI Summary for HES1

This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box. [provided by RefSeq, Jul 2008]
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Research Articles on HES1

1. High HES1 expression is associated with invasiveness and tumorigenicity of non-small cell lung cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-HES1 antibody

 Reference Product  PubMed Publications
 NOTCH1 antibody  >342 publications with HES1 and NOTCH1
 ASCL1 antibody  >52 publications with HES1 and ASCL1
 RBPJ antibody  >47 publications with HES1 and RBPJ
 NEUROG3 antibody  >23 publications with HES1 and NEUROG3
 SHH antibody  >21 publications with HES1 and SHH
 TLE1 antibody  >17 publications with HES1 and TLE1
 FANCD2 antibody  >3 publications with HES1 and FANCD2
 FANCA antibody  >2 publications with HES1 and FANCA

Pathways associated with anti-HES1 antibody

 Products by Pathway  Pathway Diagram
 ATF-2 Transcription Factor Network Pathway antibodies  ATF-2 Transcription Factor Network Pathway Diagram
 AhR Pathway antibodies  AhR Pathway Diagram
 C-MYB Transcription Factor Network Pathway antibodies  C-MYB Transcription Factor Network Pathway Diagram
 Constitutive Signaling By NOTCH1 PEST Domain Mutants Pathway antibodies  Constitutive Signaling By NOTCH1 PEST Domain Mutants Pathway Diagram
 Delta-Notch Signaling Pathway antibodies  Delta-Notch Signaling Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 FBXW7 Mutants And NOTCH1 In Cancer Pathway antibodies  FBXW7 Mutants And NOTCH1 In Cancer Pathway Diagram
 Fanconi Anemia Pathway antibodies  Fanconi Anemia Pathway Diagram
 Fanconi Anemia Pathway antibodies  Fanconi Anemia Pathway Diagram

Diseases associated with anti-HES1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >252 publications with HES1 and Neoplasms
 Carcinoma Antibodies  >85 publications with HES1 and Carcinoma
 Adenocarcinoma Antibodies  >43 publications with HES1 and Adenocarcinoma
 Necrosis Antibodies  >24 publications with HES1 and Necrosis
 Liver Diseases Antibodies  >24 publications with HES1 and Liver Diseases
 Neuroectodermal Tumors, Primitive Antibodies  >22 publications with HES1 and Neuroectodermal Tumors, Primitive
 Lung Neoplasms Antibodies  >22 publications with HES1 and Lung Neoplasms
 Inflammation Antibodies  >21 publications with HES1 and Inflammation
 Carcinoma, Non-Small-Cell Lung Antibodies  >15 publications with HES1 and Carcinoma, Non-Small-Cell Lung
 Breast Neoplasms Antibodies  >15 publications with HES1 and Breast Neoplasms

Organs/Tissues associated with anti-HES1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >221 publications with HES1 and Brain
 Nerve Antibodies  >138 publications with HES1 and Nerve
 Embryonic Tissue Antibodies  >126 publications with HES1 and Embryonic Tissue
 Bone Antibodies  >106 publications with HES1 and Bone
 Blood Antibodies  >102 publications with HES1 and Blood
 Muscle Antibodies  >59 publications with HES1 and Muscle
 Pancreas Antibodies  >56 publications with HES1 and Pancreas
 Liver Antibodies  >54 publications with HES1 and Liver
 Eye Antibodies  >53 publications with HES1 and Eye
 Vascular Antibodies  >49 publications with HES1 and Vascular
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